Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:84059
Lu DP, et al., Ligand-activated signal transduction in the 2-cell embryo. Biol Reprod. 2003 Jul;69(1):106-16
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7626332
|
|
Gene symbol:
|
Cacna1c
|
|
Gene name:
|
calcium channel, voltage-dependent, L type, alpha 1C subunit
|
|
Antibody:
|
anti-alpha1-C
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
3C,D:
embryonic day 1.0
(more  )
Specimen
3C,D:
(close  )
|
Genetic Background:
|
Swiss
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
Formaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Strong |
Regionally restricted |
3C
,
3D
|
Most intense expression in region of apical membranes of each blastomere. Essentially no expression in apposing lateral membranes between blastomeres. In most cases no expression in nucleus.
|
| TS2: second polar body |
Present |
|
3D
,
3C
|
|
Specimen
3E,F:
embryonic day 1.0
(more )
Specimen
3E,F:
(close )
|
Genetic Background:
|
Swiss
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
Formaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Strong |
Regionally restricted |
3F
,
3E
|
Most intense expression in region of apical membranes of each blastomere. Essentially no expression in apposing lateral membranes between blastomeres. Some blastomeres showed neuclear expression. No expression in nucleoli.
|
|
Analysis Tools
