Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63927
Houle M, et al., Retinoic acid regulation of Cdx1: an indirect mechanism for retinoids and vertebral specification. Mol Cell Biol. 2000 Sep;20(17):6579-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7626279
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Gene symbol:
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Cdx1
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Gene name:
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caudal type homeobox 1
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Probe:
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cdx1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A (left):
embryonic day 8.5
(more  )
Specimen
1A (left):
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
1A (left)
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Expression in ectoderm and mesoderm in primitive streak region.
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Specimen
1A (left-middle):
embryonic day 8.5
(more )
Specimen
1A (left-middle):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
1A (left-middle)
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Expression in ectoderm and mesoderm in primitive streak region.
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Specimen
1A (middle-right):
embryonic day 8.5
(more )
Specimen
1A (middle-right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
1A (middle-right)
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Expression in ectoderm and mesoderm in primitive streak region.
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Specimen
1A (right):
embryonic day 8.5
(more )
Specimen
1A (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
Regionally restricted |
1A (right)
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Expression in ectoderm and mesoderm in primitive streak region.
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Specimen
1C:
embryonic day 8.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Weak |
Regionally restricted |
1C
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Weaker expression in caudal neuroepithelium.
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Specimen
2A:
embryonic day 7.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
Regionally restricted |
2A
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Expression in ectoderm and mesoderm of primitive streak region.
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Specimen
2C:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Weak |
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2C
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Expression in primitive streak region reduced compared to wild type.
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Specimen
2E:
embryonic day 7.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Trace |
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2E
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Expression weakly detected.
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Specimen
2G:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
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2G
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Expression levels in primiitve streak region comparable to wild type.
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Specimen
2I:
embryonic day 9.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2K:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Weak |
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2K
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Authors state expression sometimes weaker than in wild type, but not consistently observed.
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| TS15: forelimb bud mesenchyme |
Weak |
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2K
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Authors state expression sometimes weaker than in wild type, but not consistently observed.
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| TS15: tail |
Trace |
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2K
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Expresion in tail bud either comparable to wild type or too week to compare.
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