Probe:	mE2F-2-D2
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: epidermis	Absent		2A/B	Expression was not detected in the epithelium at E11.5.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	2C/D	In the more developmentally advanced regions on the head and thorax, an increase in E2f2 expression was evident. Specifically, expression was detected in the underlying mesenchyme with greater levels in the epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: hair follicle	epithelial cell	Strong	Regionally restricted	2E/F	Expression was very abundant in epithelial cells from the hair follicles around the dermal papilla.

Structure	Level	Pattern	Image	Note
TS26: epidermis basal layer	Present		2G/H

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present		7A/B
TS22: intestine mesenchyme	Weak		7A/B

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Strong	Regionally restricted	7C/D	Expression level was high in the intervillus epithelium.
TS24: intestinal villus	Present	Regionally restricted	7C/D	Lower expression was detected at the tip of the forming villi.

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Present	Regionally restricted	7E	Expression was detected in the intervillus epithelium.
TS26: intestinal villus	Present	Regionally restricted	7E	Expression was localized to the lower portions of the developing villi. Staining was negligible in the apex of the villi.
TS26: intestine mesenchyme	Trace		7E

Structure	Level	Pattern	Image	Note
TS19: intestine	Trace

Structure	Level	Pattern	Image	Note
TS21: epidermis	Present	Regionally restricted		In the 13.5-dpc dorsal ectoderm, expression remains negative, whereas anterior areas in the head and ventral areas in the mid-region express low E2f2 transcript levels.
TS21: intestine	Trace

Structure	Level	Pattern	Image	Note
TS22: back skin	Absent			At 14.5 dpc, the epithelium in the back, visible as a single-cell layer, is still negative for E2f2 expression.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted		In the ventral region, which has started to stratify, expression was detected both in the epithelium and, to a lesser extent, in the underlying mesenchyme. In the dorsal regions, where the epithelium hasn't differentiated into layers, expression levels were low.
TS23: hair follicle	Present	Regionally restricted		Epithelial cells surrounding the dermal papillae have high levels of expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: epidermis basal layer	basal cell of epidermis	Present
TS24: hair follicle		Strong	Regionally restricted		High expression continues in the developing hair follicles, around dermal papillae, but not in regions of the hair sheath proximal to the epidermis, which contain the most differentiated cells in the hair shaft.

Structure	Level	Pattern	Image	Note
TS26: epidermis basal layer	Strong
TS26: hair follicle	Strong	Regionally restricted		E2f2 transcripts were very abundant around the dermal papillae.

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Strong	Regionally restricted		Expression was detected in the intervillus epithelium.
TS26: intestinal villus	Present	Regionally restricted		Expression was detected in the bottom half of the villi and negligible in the apex of the villi.
TS26: intestine mesenchyme	Trace

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Absent
TS19: intestine epithelium	Absent			Staining was not detected in pseudostratified endoderm of the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS20: intestine epithelium	Absent			Staining was not detected in pseudostratified endoderm of the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS21: intestine epithelium	Absent			Staining was not detected in pseudostratified endoderm of the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present	Regionally restricted		Expression was in pseudostratified endoderm of the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS23: epidermis basal layer	Present
TS23: epidermis suprabasal layer	Absent
TS23: hair follicle	Present	Regionally restricted		Expression pattern in epithelial cells surrounding the dermal papilla.
TS23: intestine epithelium	Present	Regionally restricted		Expression was detected in intervillus epithelium.
TS23: intestinal villus	Present	Regionally restricted		Expression was at the base, but not the apex of the villi.

Structure	Level	Pattern	Image	Note
TS26: epidermis basal layer	Present
TS26: epidermis suprabasal layer	Absent
TS26: hair follicle	Present	Regionally restricted		Expression pattern in epithelial cells surrounding the dermal papilla.
TS26: intestine epithelium	Present	Regionally restricted		Expression was detected in intervillus epithelium.
TS26: intestinal villus	Present	Regionally restricted		Expression was at the base, but not the apex of the villi.