Gene Expression Data
Assay Details
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Assay
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Reference:
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J:210169
Shinagawa T, et al., Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell. 2014 Feb 6;14(2):217-27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7623736
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Gene symbol:
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H2bc1
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Gene name:
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H2B clustered histone 1
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Antibody:
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anti-TH2B
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
1B TH2B GV oocyte:
postnatal adult
(more  )
Specimen
1B TH2B GV oocyte:
(close  )
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Genetic Background:
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involves: BALB/c
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Strong |
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1B TH2B GV oocyte
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1B TH2B GV oocyte (inset)
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Highly expressed in oocytes.
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Specimen
1B TH2B MII oocyte:
postnatal adult
(more )
Specimen
1B TH2B MII oocyte:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Strong |
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1B TH2B MII oocyte (inset)
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1B TH2B MII oocyte
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Highly expressed in oocytes.
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Specimen
1B TH2B one-cell:
embryonic day 0.5
(more )
Specimen
1B TH2B one-cell:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Strong |
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1B TH2B one-cell
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Significant expression detected in zygotes.
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Specimen
1B TH2B two-cell:
embryonic day 1.0
(more )
Specimen
1B TH2B two-cell:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1B TH2B two-cell
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Expression decreased during differentiation into blastocysts.
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Specimen
1B TH2B eight-cell:
embryonic day 2.5
(more )
Specimen
1B TH2B eight-cell:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Weak |
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1B TH2B eight-cell
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Expression decreased during differentiation into blastocysts.
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Specimen
1B TH2B Morula:
embryonic day 3.0
(more )
Specimen
1B TH2B Morula:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Weak |
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1B TH2B Morula
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Expression decreased during differentiation into blastocysts.
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Specimen
1B TH2B Blastocyst:
embryonic day 3.5
(more )
Specimen
1B TH2B Blastocyst:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS5: blastocyst |
Trace |
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1B TH2B Blastocyst
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Expression decreased during differentiation into blastocysts.
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Specimen
S1C TH2B 8-cell:
embryonic day 2.5
(more )
Specimen
S1C TH2B 8-cell:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
S1C TH2B Morula:
embryonic day 3.0
(more )
Specimen
S1C TH2B Morula:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Trace |
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S1C TH2B/DAPI Morula
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S1C TH2B Morula
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Small amount of expression retained following removal of chromatin-unbound proteins.
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Specimen
S1C TH2B Blastocyst:
embryonic day 3.5
(more )
Specimen
S1C TH2B Blastocyst:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS5: blastocyst |
Trace |
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S1C TH2B Blastocyst
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S1C TH2B/DAPI Blastocyst
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Small amount of expression retained following removal of chromatin-unbound proteins.
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Analysis Tools