Reporter:	GFP
	Direct Detection

Structure	Cell Type	Level	Pattern	Image	Note
TS28: oviduct ampulla	ciliated epithelial cell	Present	Regionally restricted	2A	Expression was detected in ciliated epithelium.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Absent		2C
TS28: spermatozoon	Absent		2C

Structure	Level	Pattern	Image	Note
TS11: node	Present		2E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: oviduct ampulla	ciliated epithelial cell	Present	Regionally restricted	2F , 2H	Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: choroid plexus	ciliated epithelial cell	Present	Regionally restricted	2N , 2L	Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: trachea	ciliated epithelial cell	Weak	Regionally restricted	2Q , 2O	Expression was detected in the cilia, which were identified by anti-acetylated tubulin. No GFP fluorescence was observed in nonciliated cells, and no GFP fluorescence was seen in the cytoplasm of ciliated cells.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS10: node	Weak	Regionally restricted	3B	Faint GFP fluorescence was visible as small dots in the ventral node cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS10: node	ciliated cell	Present		3F , 3D
TS10: node		Present	Regionally restricted	3D , 3E	GFP fluorescence was detected only in ciliated cells in the node. In a deeper section of cell bodies in the ventral node, no GFP fluorescence was identified.

Structure	Cell Type	Level	Pattern	Image	Note
TS11: node	ciliated cell	Present	Regionally restricted	3H , 3I , 3K , 3J	GFP fluorescence was found in a centrally located subset of node monocilia, which were identified by anti-acetylated tubulin. No left-right asymmetry was noted in the distribution of GFP fluorescing cilia. A second subset of node monocilia does not show GFP fluorescence, predominantly found at the left, right and anterior margins of the node (31-33% of all cilia).

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS11: node	ciliated cell	Present	Regionally restricted	5E , 5C , 5B , 5H , 5F , 5I	GFP fluorescence was detected in central node monocilia. Central, motile monocilia were labeled for both Pkd2 and Dnah11-GFP reporter. Peripheral, immotile cilia were labeled exclusively for Pkd2.