Nkx2-2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:85847 Kokubu C, et al., Undulated short-tail deletion mutation in the mouse ablates Pax1 and leads to ectopic activation of neighboring Nkx2-2 in domains that normally express Pax1. Genetics. 2003 Sep;165(1):299-307
Assay type:	RNA in situ
MGI Accession ID:	MGI:7620536
Gene symbol:	Nkx2-2
Gene name:	NK2 homeobox 2

Probe:	Nkx2-2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 4A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4A	Expression was detected in the ventral region of the neural tube adjacent to the floor plate.

Specimen 4B: embryonic day 10.5; Pax1^tm1Neu/Pax1^tm1Neu (more

)

Structure	Level	Image	Note
TS17: neural tube	Present	4B	Normal neural tube expression was not affected in the mutant.
TS17: limb bud	Absent	4B	No ectopic expression was observed in the null mutant.
TS17: sclerotome	Absent	4B	No ectopic expression was observed in the null mutant.

Specimen 4C: embryonic day 10.5; Pax1^Un-s/Pax1⁺ (more

)

Structure	Level	Image	Note
TS17: limb bud	Present	4C	Ectopic expression was observed in the mutant.
TS17: sclerotome	Present	4C	Ectopic expression was observed in the mutant.
TS17: neural tube	Present	4C	Normal neural tube expression was not affected in the mutant.

Specimen 4D: embryonic day 10.5; Pax1^Un-s/Pax1^Un-s (more

)

Structure	Level	Image	Note
TS17: limb bud	Present	4D	Ectopic expression was observed in the mutant.
TS17: sclerotome	Present	4D	Ectopic expression was observed in the mutant.
TS17: neural tube	Present	4D	Normal neural tube expression was not affected in the mutant.
TS17: branchial pouch endoderm	Absent	4D	There was no ectopic expression detected in the pharyngeal pouch endoderm.

Specimen 4F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4F	Expression was detected in the ventral region of the neural tube adjacent to the floor plate.

Specimen 4G: embryonic day 10.5; Pax1^tm1Neu/Pax1^tm1Neu (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		4G	Normal neural tube expression was not affected in the mutant.
TS17: sclerotome	Absent		4G	No ectopic expression was observed in the null mutant.

Specimen 4H: embryonic day 10.5; Pax1^Un-s/Pax1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Present		4H	Ectopic expression was observed in the mutant.
TS17: neural tube	Present		4H	Normal neural tube expression was not affected in the mutant.

Specimen 4I: embryonic day 10.5; Pax1^Un-s/Pax1^Un-s (more

)

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Present		4I	Ectopic expression was observed in the mutant.
TS17: neural tube	Present		4I	Normal neural tube expression was not affected in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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