Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:60735
Bumsted KM, et al., Dorsal retinal pigment epithelium differentiates as neural retina in the microphthalmia (mi/mi) mouse. Invest Ophthalmol Vis Sci. 2000 Mar;41(3):903-8
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7619953
|
|
Gene symbol:
|
Mitf
|
|
Gene name:
|
melanogenesis associated transcription factor
|
|
Antibody:
|
Anti-mi
|
|
Detection system:
|
Secondary antibody coupled to Texas Red
|
|
|
Results
|
Specimen
1C:
embryonic day 13.5-14.5
(more )
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5-14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
1D:
(close )
|
Genetic Background:
|
B6C3Fe-a/a
|
|
Mutant Allele(s):
|
MitfMi/MitfMi
|
|
Age:
|
embryonic day 13.5-14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pigmented retinal epithelium |
Present |
Regionally restricted |
1D
|
In the mutant, expression was not found in the dorsal expanded region of the retinal pigmented epithelium (RPE), but was observed in only in regions where the RPE remained a monolayer.
|
| TS21: neural retina |
Absent |
|
1D
|
|
|