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Gene Expression Data
Assay Details
Assay
Reference: J:213418 Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
Assay type: RNA in situ
MGI Accession ID: MGI:7615141
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Probe: Col2a1 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3I: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb digit 1 mesenchyme Present Regionally restricted 3I Expressed in skeletal condensations.
TS20: forelimb digit 2 mesenchyme Present Regionally restricted 3I Expressed in skeletal condensations.
TS20: forelimb digit 3 mesenchyme Present Regionally restricted 3I Expressed in skeletal condensations.
TS20: forelimb digit 4 mesenchyme Present Regionally restricted 3I Expressed in skeletal condensations.
TS20: forelimb digit 5 mesenchyme Present Regionally restricted 3I Expressed in skeletal condensations.
TS20: radius-ulna pre-cartilage condensation Present 3I
Specimen 3J: embryonic day 12.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Structure Level Pattern Image Note
TS20: forelimb digit 1 mesenchyme Present Regionally restricted 3J Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 2 mesenchyme Present Regionally restricted 3J Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 3 mesenchyme Present Regionally restricted 3J Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 4 mesenchyme Present Regionally restricted 3J Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 5 mesenchyme Present Regionally restricted 3J Expressed in skeletal condensations, similar as in the control.
TS20: radius-ulna pre-cartilage condensation Present 3J Similar as in the control.
Specimen 3K: embryonic day 12.5; Kif7tm1.2Hui/Kif7tm1.2Hui (more )
Structure Level Pattern Image Note
TS20: radius-ulna pre-cartilage condensation Present 3K Similar as in the control.
TS20: forelimb digit 1 mesenchyme Present 3K Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 2 mesenchyme Present 3K Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 3 mesenchyme Present 3K Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 4 mesenchyme Present 3K Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 5 mesenchyme Present 3K Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit mesenchyme Present 3K Extra digit forming in the anterior forelimb in the mutant.
Specimen 3L: embryonic day 12.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui, Kif7tm1.2Hui/Kif7tm1.2Hui (more )
Structure Level Pattern Image Note
TS20: forelimb digit 2 mesenchyme Present 3L Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 3 mesenchyme Present 3L Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 4 mesenchyme Present 3L Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit 5 mesenchyme Present 3L Expressed in skeletal condensations, similar as in the control.
TS20: forelimb digit mesenchyme Present Regionally restricted 3L Lack of chondrogenesis for digit 1 was seen.
TS20: radius-ulna pre-cartilage condensation Present Regionally restricted 3L Lack of chondrogenesis for radius was seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/14/2026
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