Ube4b Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:182252 Mammen AL, et al., A novel conserved isoform of the ubiquitin ligase UFD2a/UBE4B is expressed exclusively in mature striated muscle cells. PLoS One. 2011;6(12):e28861
Assay type:	Western blot
MGI Accession ID:	MGI:7612252
Gene symbol:	Ube4b
Gene name:	ubiquitination factor E4B

Antibody:

anti-UFD2a

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Size Not Specified (d)	163 kDa (e)	Genetic Background	Mutant Allele(s)	Sex	Note
E14	E14.0 (a)	TS22: skeletal muscle	Absent (c)	Present	Absent (c)	C57BL/6J		Not Specified	pooled gastrocnemius, soleus, and quadriceps muscles
E16	E16.0 (a)	TS24: skeletal muscle	Absent	Present	Absent	C57BL/6J		Not Specified	pooled gastrocnemius, soleus, and quadriceps muscles
E18	E18.0	TS26: skeletal muscle	Trace (f)	Present	Absent (g)	C57BL/6J		Not Specified	pooled gastrocnemius, soleus, and quadriceps muscles
P1	P1	TS27: skeletal muscle	Present (h)	Present	Present (h)	C57BL/6J		Not Specified	pooled gastrocnemius, soleus, and quadriceps muscles
P7	P7	TS28: skeletal muscle	Weak (i)	Present	Present	C57BL/6J		Not Specified	pooled gastrocnemius, soleus, and quadriceps muscles

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) UFD2a-7
(c) No expression at E14.
(d) UFD2a
(e) UFD2a-7/7a
(f) USD2a-7 not detecable until E18.
(g) UFD2a-7/7a was expressed after USD2a-7.
(h) Both forms detected at P1, as a doublet.
(i) Expression reduced.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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