Antibody:	MEC 13.3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS17: yolk sac blood vessel	blood vessel endothelial cell	Present		2C WT	An organized vascular network of branching vessels that were lined with positive endothelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: yolk sac blood vessel	blood vessel endothelial cell	Present		2C m/m	Remodeling process did not take place and the vessels remained in the honeycomb-like pattern in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: head blood vessel	blood vessel endothelial cell	Present		3A WT	The branched cephalic large vessels and capillary networks were readily distinguishable in the dorsal midbrain.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: head blood vessel	blood vessel endothelial cell	Present		3A m/m	The cephalic plexus in mutant embryos failed to remodel into a branched vascular network and instead remained as a plexus with minor sprouting.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	aortic endothelial cell	Present		3B WT	A continuous dorsal aorta along the length of the embryo.
TS15: cardinal vein	vein endothelial cell	Present		3B WT	A well-defined cardinal vein from head to heart.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	aortic endothelial cell	Present		3B m/m	The edges of the dorsal aorta were not clearly delineated in the mutant.
TS15: cardinal vein	vein endothelial cell	Present		3B m/m	The anterior cardinal vein appeared deformed and unusually close to the dorsal aorta.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	aortic endothelial cell	Present		3C WT
TS15: cardinal vein	vein endothelial cell	Present		3C WT

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	aortic endothelial cell	Present		3C m/m	The dorsal aortas were enlarged and irregular in shape on both sides.
TS15: cardinal vein	vein endothelial cell	Present		3C m/m	One of the anterior cardinal veins was severely deformed and unusually close to the dorsal aorta.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: brain blood vessel	blood vessel endothelial cell	Present		3E WT	Extensive branching in the vasculature was seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: brain blood vessel	blood vessel endothelial cell	Present		3E m/m	The mutant still displayed the honeycomb-like pattern of primary capillary plexus.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: dorsal aorta	aortic endothelial cell	Present		4C WT
TS19: cardinal vein	vein endothelial cell	Present		4C WT

Structure	Cell Type	Level	Pattern	Image	Note
TS19: cardinal vein	vein endothelial cell	Present		4C m/m	Dilated cardinal vein and arteriovenous malformation resulting from fusion of the dorsal aorta with the common cardinal vein were seen.
TS19: dorsal aorta	aortic endothelial cell	Present		4C m/m	An arteriovenous malformation resulting from fusion of the dorsal aorta with the common cardinal vein was seen.