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Gene Expression Data
Assay Details
Assay
Reference: J:293624 Lee S, et al., Cleft lip and cleft palate in Esrp1 knockout mice is associated with alterations in epithelial-mesenchymal crosstalk. Development. 2020 Apr 30;147(21):dev187369
Assay type: RT-PCR
MGI Accession ID: MGI:7607154
Gene symbol: Fgfr2
Gene name: fibroblast growth factor receptor 2
Probe: Fgfr2-pF, Fgfr2-pR
Assay notes: RT-PCR validations of changes in splicing of mutually exclusive exons IIIb and IIIc of Fgfr2. Products containing each exon were distinguished by restriction digests with AvaI and HincII, which cut products containing exon IIIb and IIIc, respectively.
Results
Image: 4E Fgfr2 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
U WT E12.0 TS19: facial prominence ectoderm Present (b) Weak 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Not Specified Uncut
A WT E12.0 TS19: facial prominence ectoderm Present (d) Weak (e) 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Not Specified Cut with AvaI
H WT E12.0 TS19: facial prominence ectoderm Present (d) Weak (e) 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Not Specified Cut with HincII
U E1KO E12.0 TS19: facial prominence ectoderm Present (f) Present 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Esrp1tm1.2Rpc/Esrp1tm1.2RpcNot Specified Uncut
A E1KO E12.0 TS19: facial prominence ectoderm Present (g) Present (e) 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Esrp1tm1.2Rpc/Esrp1tm1.2RpcNot Specified Cut with AvaI
H E1KO E12.0 TS19: facial prominence ectoderm Present (g) Present (e) 0.3 µg; total RNA involves: 129S4/SvJae * C57BL/6 Esrp1tm1.2Rpc/Esrp1tm1.2RpcNot Specified Cut with HincII
Notes:
(a) exon IIIb
(b) 98% exon IIIb
(c) exon IIIc
(d) Lane contained digestion products. 98% exon IIIb
(e) Lane contained digestion products.
(f) 34% exon IIIb
(g) Lane contained digestion products. 34% exon IIIb
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory