Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7606961
|
|
Gene symbol:
|
Prox1
|
|
Gene name:
|
prospero homeobox 1
|
|
Probe:
|
Prox1 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Assay notes:
|
A signal amplification step (tyramide signal amplification) was used.
|
|
|
Results
|
Specimen
8A:
embryonic day 14.5
(more  )
Specimen
8A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hippocampus |
Weak |
|
8A
|
Expressed in the hippocampal neuroepithelium.
|
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx2tm1Pgr/Emx2tm1Pgr
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
Specimen
8C:
embryonic day 18.5
(more )
Specimen
8C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Strong |
Regionally restricted |
8C
|
Expressed in the tangential migratory stream and in the tertiary matrix. The germinative ventricular and subventricular zones were no longer labeled.
|
Specimen
8D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Emx2tm1Pgr/Emx2tm1Pgr
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Strong |
Regionally restricted |
8D
|
Expressed in ventricular and subventricular zones. No positive cells were seen at this age in the mutant radial migration stream, but formed abnormally large, strongly expressing groups in the tangential migration stream.
|
|
Analysis Tools
