Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174498
Oldekamp J, et al., bHLH gene expression in the Emx2-deficient dentate gyrus reveals defective granule cells and absence of migrating precursors. Cereb Cortex. 2004 Sep;14(9):1045-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7606951
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Antibody:
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anti-Ki67
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2B:
embryonic day 18.5
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
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2B
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Positive proliferating cells could be found in every subregion, including the ventricular and subventricular layers, migratory pathway and the tertiary matrix.
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| TS26: hippocampal fissure |
Present |
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2B
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Specimen
2D/E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dentate gyrus |
Present |
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2D
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2E
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Dramatically decreased expression. Mainly expressed in ventricular and subventricular zones, and some scattered ones in the radial and tangential migration streams.
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| TS26: hippocampal fissure |
Present |
Single cells |
2E
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2D
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Very sparse proliferating cells.
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Analysis Tools
