Gene Expression Data
Assay Details
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Assay
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Reference:
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J:214999
Pearton DJ, et al., Elf5 counteracts precocious trophoblast differentiation by maintaining Sox2 and 3 and inhibiting Hand1 expression. Dev Biol. 2014 Aug 15;392(2):344-57
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7606210
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Probe:
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Sox2 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5F:
embryonic day 6.5
(more  )
Specimen
5F:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: extraembryonic ectoderm |
Present |
Regionally restricted |
5F
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Expression restricted to proximal part of extraembryonic ectoderm at E6.5.
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| TS9: embryo ectoderm |
Present |
Regionally restricted |
5F
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Expression also commencing in anterior neuroectoderm.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Elf5tm1Plp/Elf5tm1Plp
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: trophectoderm |
Absent |
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5G
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Trophoblast expression lost in mutant embryos.
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| TS9: embryo ectoderm |
Present |
Regionally restricted |
5G
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Expression continued in what is likely to be prostective neurectoderm.
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Analysis Tools
