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Gene Expression Data
Assay Details
Assay
Reference: J:334252 Zapata G, et al., Generation of a mouse model of the neurodevelopmental disorder with dysmorphic facies and distal limb anomalies syndrome. Hum Mol Genet. 2022 Oct 10;31(20):3405-3421
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7595951
Gene symbol: Aif1
Gene name: allograft inflammatory factor 1
Results
Specimen E13.5 Ctrl not shown: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex mature microglial cell Present Expression in activated microglia.
Specimen E13.5 Het not shown: embryonic day 13.5; Bptftm1.1Cwu/Bptf+ (more )
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex mature microglial cell Present
Specimen E13.5 cKO not shown: embryonic day 13.5; Bptftm1.1Cwu/Bptftm1.1Cwu, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex mature microglial cell Present No signficant changes compared to control and heterozygote.
Specimen 3A Ctrl: embryonic day 15.5 (more )
Note: Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex ventricular layer mature microglial cell Present 3A Ctrl
Specimen 3A Het: embryonic day 15.5; Bptftm1.1Cwu/Bptf+ (more )
Note: Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex ventricular layer mature microglial cell Present 3A Het
Specimen 3A cKO: embryonic day 15.5; Bptftm1.1Cwu/Bptftm1.1Cwu, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex intermediate zone mature microglial cell Present 3A cKO Levels within cortical plate and intermediate zone remained unchanged compared to control.
TS23: cortical plate mature microglial cell Present 3A cKO Levels within cortical plate and intermediate zone remained unchanged compared to control.
TS23: cerebral cortex ventricular layer mature microglial cell Present 3A cKO More microglia within ventricular zone compared to control.
Specimen 3D Ctrl: postnatal day 2 (more )
Note: Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS27: neocortex mature microglial cell Trace 3D Ctrl Increased expressing cells not observed in control.
Specimen 3D Het: postnatal day 2; Bptftm1.1Cwu/Bptf+ (more )
Note: Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS27: neocortex mature microglial cell Trace 3D Het
Specimen 3D cKO: postnatal day 2; Bptftm1.1Cwu/Bptftm1.1Cwu, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Casp3 (assay MGI:7595949).
Structure Cell Type Level Pattern Image Note
TS27: neocortex mature microglial cell Present 3D inset , 3D cKO 10-fold increased in expressing cells compared to control.
Specimen P7 Ctrl not shown: postnatal day 7 (more )
Structure Cell Type Level Pattern Image Note
TS28: neocortex mature microglial cell Trace
Specimen P7 Het not shown: postnatal day 7; Bptftm1.1Cwu/Bptf+ (more )
Structure Level Pattern Image Note
TS28: neocortex Trace
Specimen P7 cKO not shown: postnatal day 7; Bptftm1.1Cwu/Bptftm1.1Cwu, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: neocortex Present Number of expressing cells significantly increased compared to both other genotypes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory