Gene Expression Data
Assay Details
Assay
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Reference:
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J:146220
Maclean G, et al., Genetic disruption of CYP26B1 severely affects development of neural crest derived head structures, but does not compromise hindbrain patterning. Dev Dyn. 2009 Mar;238(3):732-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7595648
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Gene symbol:
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Crabp1
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Gene name:
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cellular retinoic acid binding protein I
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Probe:
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Crabp1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
6I:
embryonic day 9.5
(more )
Specimen
6I:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: 2nd branchial arch |
migratory neural crest cell |
Present |
Regionally restricted |
6I
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Expression was detected in anterior second branchial arch.
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TS15: face |
migratory cranial neural crest cell |
Present |
Regionally restricted |
6I
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Expression was detected in migratory and early-post migratory neural crest cells at all craniofacial levels.
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TS15: migrating neural crest |
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Present |
Regionally restricted |
6I
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Expression was detected in streams of cells connecting the post-otic hindbrain to the arches.
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Specimen
6J:
embryonic day 9.5
(more )
Specimen
6J:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: 2nd branchial arch |
migratory neural crest cell |
Present |
Regionally restricted |
6J
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Expression was detected in anterior second branchial arch.
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TS15: face |
migratory cranial neural crest cell |
Present |
Regionally restricted |
6J
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Expression was detected in migratory and early-post migratory neural crest cells at all craniofacial levels.
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TS15: migrating neural crest |
|
Present |
Regionally restricted |
6J
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Expression was detected in streams of cells connecting the post-otic hindbrain to the arches.
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Specimen
6K:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Cyp26b1tm1.1Ptk/Cyp26b1tm1.1Ptk
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: 2nd branchial arch |
migratory neural crest cell |
Present |
Regionally restricted |
6K
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No difference in expression was observed between Cyp26b1-/- and wild type embryos.
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TS15: face |
migratory neural crest cell |
Present |
Regionally restricted |
6K
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No difference in expression was observed between Cyp26b1-/- and wild type embryos.
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TS15: migrating neural crest |
|
Present |
Regionally restricted |
6K
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The streams of cells connecting the post-otic hindbrain to the arches were highly disorganized in Cyp26b1-/- compared with wild type.
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Specimen
6L:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Cyp26b1tm1.1Ptk/Cyp26b1tm1.1Ptk
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: 2nd branchial arch |
migratory neural crest cell |
Present |
Regionally restricted |
6L
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No difference in expression was observed between Cyp26b1-/- and wild type embryos.
|
TS15: face |
migratory neural crest cell |
Present |
Regionally restricted |
6L
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No difference in expression was observed between Cyp26b1-/- and wild type embryos.
|
TS15: migrating neural crest |
|
Present |
Regionally restricted |
6L
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The streams of cells connecting the post-otic hindbrain to the arches were highly disorganized in Cyp26b1-/- compared with wild type.
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Specimen
not shown wild type:
embryonic day 8.5-9.0
(more )
Specimen
not shown wild type:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 8.5-9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS13: neural crest |
migratory neural crest cell |
Present |
Regionally restricted |
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Expression was detected in migratory streams from r2 and r4.
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Specimen
not shown Cyp26b1-/-:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Cyp26b1tm1.1Ptk/Cyp26b1tm1.1Ptk
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Age:
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embryonic day 8.5-9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS13: neural crest |
migratory neural crest cell |
Present |
Regionally restricted |
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No difference in migratory stream expression from r2 and r4 was observed between Cyp26b1-/- and wild type embryos.
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