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Gene Expression Data
Assay Details
Assay
Reference: J:244466 Karpanen T, et al., An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation. Circ Res. 2017 Apr 14;120(8):1263-1275
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7595504
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: Anti-Prox1 (102-PA32)
Assay notes: Two Prox1 antibodies were described, but the one used in this assay was not detailed. Anti-Prox-1 (11-002) MGI:5555854 may have been used in this assay.
Results
Specimen 7A: embryonic day 11.0 (more )
Note: Triple labeled: green - Prox1; blue - Flt4 (assay MGI:7595500); red - Pecam1 (assay MGI:7595501).
Structure Level Pattern Image Note
TS18: lymphatic system Present Regionally restricted 7A Expression in lymphatic endothelial cells.
Specimen 7B: embryonic day 11.0; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Triple labeled: green - Prox1; blue - Flt4 (assay MGI:7595500); red - Pecam1 (assay MGI:7595501).
Structure Level Pattern Image Note
TS18: lymphatic system Present Regionally restricted 7B No difference in formation of first lymphatic structures between wild-type and mutant.
Specimen 7C: embryonic day 12.5 (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7C Expression in lymphatic endothelial cells.
Specimen 7D: embryonic day 12.5; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7D No difference in formation of first lymphatic structures between wild-type and mutant.
Specimen 7E: embryonic day 12.5 (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7E Accumulation of expressing cells at site of lymphovenous connection site.
Specimen 7F: embryonic day 12.5; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7F Typical clustering of cells did not occur in mutant.
Specimen 7G: embryonic day 12.5 (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7G Tight association of lymphatic and venous endothelium.
Specimen 7H: embryonic day 12.5; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 7H Tight association of lymphatic and venous endothelium not observed in mutant..
Specimen 7I: embryonic day 13.5 (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted 7I Tight association of lymphatic and venous endothelium.
Specimen 7J: embryonic day 13.5; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Double labeled: green - Prox1; blue - Flt4 (assay MGI:7595500).
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted 7J Tight association of lymphatic and venous endothelium not observed in mutant..
Specimen 8A,B,E: embryonic day 18.5 (more )
Note: Triple labeled: blue - Prox1; green - Flt4 (assay MGI:7595500); red - Pecam1 (assay MGI:7595501).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted 8E , 8B , 8A Remodeling of lymphatic vessels was apparent, including formation of lymphatic valve structures expressing all 3 markers. Expression downregulated in collecting lymphatic vessels.
Specimen 8F,G,J: embryonic day 18.5; Svep1tm1a(EUCOMM)Hmgu/Svep1tm1a(EUCOMM)Hmgu (more )
Note: Triple labeled: blue - Prox1; green - Flt4 (assay MGI:7595500); red - Pecam1 (assay MGI:7595501).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted 8J , 8G , 8F Lymphatic valve structures were absent in mesentery. Lymphatic vessels were considerably smaller and retained higher levels of expression than wild-type, and were not lumenized,
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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