Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:220849
Achilleos A, et al., MBTPS1/SKI-1/S1P proprotein convertase is required for ECM signaling and axial elongation during somitogenesis and vertebral development. Hum Mol Genet. 2015 May 15;24(10):2884-98
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7593905
|
Gene symbol:
|
Mesp2
|
Gene name:
|
mesoderm posterior 2
|
Probe:
|
Mesp2 probe
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
5M:
embryonic day 11.5
(more )
Specimen
5M:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Present |
Regionally restricted |
5M
|
Expression in bilateral single bands corresponding with anterior half or border of next presumptive somite to form.
|
Specimen
5N:
embryonic day 11.5
(more )
Specimen
5N:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Present |
Regionally restricted |
5N
|
Expression in bilateral single bands corresponding with anterior half or border of next presumptive somite to form.
|
Specimen
5O:
embryonic day 11.5
(more )
Specimen
5O:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Present |
Regionally restricted |
5O
|
Expression in bilateral single bands corresponding with anterior half or border of next presumptive somite to form.
|
Specimen
5P:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Mutant Allele(s):
|
Mbtps1tm1Jdh/Mbtps1tm1Jdh Tg(Col1a1-cre)1Bek/0
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Weak |
Regionally restricted |
5P
|
Expression levels substantially reduced and expression pattern disrupted consistent with abnormal somite segmentation.
|
Specimen
5Q:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Mutant Allele(s):
|
Mbtps1tm1Jdh/Mbtps1tm1Jdh Tg(Col1a1-cre)1Bek/0
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Weak |
Regionally restricted |
5Q
|
Expression levels substantially reduced and expression pattern disrupted consistent with abnormal somite segmentation.
|
Specimen
5R:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * CD-1
|
Mutant Allele(s):
|
Mbtps1tm1Jdh/Mbtps1tm1Jdh Tg(Col1a1-cre)1Bek/0
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: unsegmented mesenchyme |
Weak |
Regionally restricted |
5R
|
Expression levels substantially reduced and expression pattern disrupted consistent with abnormal somite segmentation.
|
|