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Gene Expression Data
Assay Details
Assay
Reference: J:151457 Nelson BR, et al., Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development. Dev Dyn. 2009 Feb 2;238(9):2163-2178
Assay type: RNA in situ
MGI Accession ID: MGI:7593723
Gene symbol: Dll1
Gene name: delta like canonical Notch ligand 1
Probe: MGC clone 62324
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: neural retina Strong Single cells 1A Strong expression was detected in single cells in the neurogenic region of the neural retina.
Specimen 1B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: neural retina Strong Regionally restricted 1B Strong expression was detected in the neurogenic zone.
Specimen 1C: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: neural retina Strong Regionally restricted 1C Strong expression was detected in the neurogenic zone.
TS25: ciliary body Present Regionally restricted 1C Expression was detected in the peripheral non-neurogenic ciliary epithelium.
Specimen 2B: embryonic day 14.5 (more )
Note: Double labeled: black - Dll1; red - Tubb3 (assay MGI:7593765).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2B center , 2B left Dll1 expression in the neural retina doesn't co-label Tubb3-positive neurons in the retinal ganglion cell layer, nor in Tubb3-positive newborn neurons in the progenitor zone.
Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: black - Dll1; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS22: neural retina retinal progenitor cell Present 2E center , 2E left Dll1-expressing cells can incorporate BrdU.
Specimen 3A: embryonic day 17.5 (more )
Note: Double labeled: black - Dll1; red - PH3.
Structure Level Pattern Image Note
TS25: neural retina Present Regionally restricted 3A middle , 3A top The majority of PH3+ mitotic progenitors at the apical surface do not co-express Dll1.
Specimen 3D: embryonic day 18.5 (more )
Note: Double labeled: black - Dll1; red - Pcna (assay MGI:7593766).
Structure Level Pattern Image Note
TS26: retina nuclear layer Present 3D center left , 3D left middle , 3D left top , 3D center right Dll1 expression overlaps with PCNA immunolabeling in the neuroblast layer.
TS26: retina ganglion cell layer Absent 3D left middle , 3D center left , 3D center right , 3D left top
Specimen 5A: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina Present 5A
Specimen 5A': postnatal day 0; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS27: retina Weak 5A' Expression levels were decreased in the Ascl1-/- retina compared with normal expression levels in the Ascl1+/+ retina.
Specimen S1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present S1A
TS20: diencephalon Present S1A
Specimen S1A inset: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted S1A inset Expression was detected in the rostral spinal cord.
Specimen S2A: embryonic day 17.5 (more )
Note: Double labeled: black - Dll1; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS25: neural retina retinal progenitor cell Present S2A left , S2A center Dll1-expressing cells can incorporate BrdU.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory