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Gene Expression Data
Assay Details
Assay
Reference: J:237223 Torii H, et al., Septin7 regulates inner ear formation at an early developmental stage. Dev Biol. 2016 Nov 15;419(2):217-228
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7582026
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: Anti-Sox2 (Y-17; sc-17320)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 1C/E: embryonic day 9.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Septin7 (assay MGI:7582021).
Structure Level Pattern Image Note
TS15: otocyst Present 1E , 1C Expressed in presumptive sensory epithelial cells.
Specimen 1D/F: embryonic day 9.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Septin7 (assay MGI:7582021).
Structure Level Pattern Image Note
TS15: otocyst Present 1F , 1D Expressed in presumptive sensory epithelial cells, similar as in the control.
Specimen 5C/E: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Septin7 (assay MGI:7582021).
Structure Level Pattern Image Note
TS21: inner ear epithelium Present 5E , 5C Expressed in presumptive sensory epithelial cells.
Specimen 5D/F: embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Septin7 (assay MGI:7582021).
Structure Level Pattern Image Note
TS21: inner ear epithelium Present 5F , 5D Positive presumptive sensory epithelial cells were still evident, although the area was smaller than in control mice.
Specimen 6A/C: embryonic day 18.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Myo6 (assay MGI:7582031).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium ear hair cell Present 6A , 6C Coexpressed with Myo6.
TS26: inner ear epithelium auditory epithelial supporting cell Present 6C , 6A Sox2+Myo6- cells.
Specimen 6E: embryonic day 18.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Level Pattern Image Note
TS26: inner ear epithelium Present 6E Innervation of auditory nerves (Tubb3+) was seen.
Specimen 6F/H: embryonic day 18.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Myo6 (assay MGI:7582031).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium ear hair cell Present Single cells 6F , 6H Coexpressed with Myo6 in some cells, even though the gross morphology of the inner ears was highly hypoplastic.
TS26: inner ear epithelium auditory epithelial supporting cell Present Single cells 6H , 6F Sox2+Myo6- cells.
Specimen 6I/K: embryonic day 18.5; Foxg1tm1(cre)Skm/Foxg1+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Level Pattern Image Note
TS26: inner ear epithelium Present 6I , 6K Innervation of auditory nerves (Tubb3+) was seen.
Specimen 7C/G: embryonic day 15.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Cell Type Level Pattern Image Note
TS23: cochlea epithelium auditory epithelial cell Present 7G , 7C Expressed in sensory epithelia cells.
Specimen 7D/H: embryonic day 15.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Cell Type Level Pattern Image Note
TS23: inner ear epithelium auditory epithelial cell Present 7D , 7H Expressed in sensory epithelia cells, similar as in the control.
Specimen 9E: embryonic day 18.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium auditory epithelial cell Present 9E Expressed in sensory epithelia cells.
Specimen 9F: embryonic day 18.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Tubb3 (assay MGI:7582032).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium auditory epithelial cell Present 9F Expressed in sensory epithelia cells, similar as in the control.
Specimen 9G/K: embryonic day 18.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7+ (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Myo6 (assay MGI:7582031).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium ear hair cell Present 9K , 9G Coexpressed with Myo6.
TS26: inner ear epithelium auditory epithelial supporting cell Present 9G , 9K Sox2+Myo6- cells.
Specimen 9H/L: embryonic day 18.5; Emx2tm2(cre)Sia/Emx2+, Septin7tm1Ksh/Septin7tm1Ksh (more )
Note: Conditional mutant. Double labeled: red - Sox2; green - Myo6 (assay MGI:7582031).
Structure Cell Type Level Pattern Image Note
TS26: inner ear epithelium ear hair cell Present 9H , 9L Coexpressed with Myo6, similar as in the control.
TS26: inner ear epithelium auditory epithelial supporting cell Present 9L , 9H Sox2+Myo6- cells, similar as in the control.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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