Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:309045
Garcia-Guillen IM, et al., Netrin-1/DCC Signaling Differentially Regulates the Migration of Pax7, Nkx6.1, Irx2, Otp, and Otx2 Cell Populations in the Developing Interpeduncular Nucleus. Front Cell Dev Biol. 2020;8:588851
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7581072
|
|
Gene symbol:
|
Irx2
|
|
Gene name:
|
Iroquois homeobox 2
|
|
Probe:
|
MGC clone 27958
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2C:
embryonic day 18.5
(more  )
Note: medial sagittal section
Specimen
2C:
(close  )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
medial sagittal section
|
Specimen
2D:
embryonic day 18.5; Dcctm1Wbg/ Dcctm1Wbg
(more )
Note: medial sagittal section
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Dcctm1Wbg/Dcctm1Wbg
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
medial sagittal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Absent |
|
2D
|
No expression in the interpeduncular rostral region.
|
| TS26: hindbrain |
Strong |
Regionally restricted |
2D
|
Expressed in ectopic patches of cells that had ceased to migrate toward the interpeduncular nucleus, and in aberrant band of positive cells that appeared not to have completed the migratory process through the mantle, accumulated densely in the corresponding periventricular stratum.
|
Specimen
not shown:
embryonic day 18.5
(more )
Note: medio-lateral section
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
medio-lateral section
|
Specimen
not shown:
embryonic day 18.5; Dcctm1Wbg/ Dcctm1Wbg
(more )
Note: medio-lateral section
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Dcctm1Wbg/Dcctm1Wbg
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
medio-lateral section
|
|
Analysis Tools
