Gene Expression Data
Assay Details
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Assay
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Reference:
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J:309045
Garcia-Guillen IM, et al., Netrin-1/DCC Signaling Differentially Regulates the Migration of Pax7, Nkx6.1, Irx2, Otp, and Otx2 Cell Populations in the Developing Interpeduncular Nucleus. Front Cell Dev Biol. 2020;8:588851
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7581071
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Gene symbol:
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Nkx6-1
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Gene name:
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NK6 homeobox 1
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Probe:
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Nkx6.1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 18.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Dcctm1Wbg/Dcctm1Wbg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Trace |
Regionally restricted |
2B
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Expression in the prodromal region was almost absent, with some cells that had reached their correct location.
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| TS26: midbrain-hindbrain junction |
Present |
Patchy |
2B
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Expressed in anomalous ectopic patches of cells that had ceased to migrate toward the interpeduncular nucleus.
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Analysis Tools
