Gene Expression Data
Assay Details
Assay
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Reference:
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J:310453
Kyrousi C, et al., Mcidas and GemC1 are key regulators for the generation of multiciliated ependymal cells in the adult neurogenic niche. Development. 2015 Nov 1;142(21):3661-74
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7580574
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Gene symbol:
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Mcidas
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Gene name:
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multiciliate differentiation and DNA synthesis associated cell cycle protein
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Antibody:
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anti-hIdasAb2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1E:
embryonic day 16.5
(more )
Note: Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
Specimen
1E:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
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Age:
|
embryonic day 16.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Present |
Regionally restricted |
1E'
,
1E''
,
1E
|
Expression is in the periventricular cell layer. There is about 50% co-localization.
|
Specimen
1F:
embryonic day 18.5
(more )
Note: Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
Specimen
1F:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: telencephalon |
Present |
Regionally restricted |
1F''
,
1F
,
1F'
|
Expression is in the periventricular cell layer. The majority of cells are double labeled.
|
Specimen
1G:
embryonic day 18.5
(more )
Note: Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
Specimen
1G:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
|
Specimen
1H:
embryonic day 18.5
(more )
Note: Double labeled: green - Mcidas; red - Tubb3 (assay MGI:7580576).
Specimen
1H:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcidas; red - Tubb3 (assay MGI:7580576).
|
Specimen
S2A:
postnatal day 0
(more )
Note: Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
Specimen
S2A:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 0
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: telencephalon |
Present |
Regionally restricted |
S2A''
,
S2A
|
Expression is in the anterior-dorsal part of the lateral wall.
|
Specimen
S2B:
postnatal day 15
(more )
Note: Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
Specimen
S2B:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 15
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
S2B
,
S2B''
|
Expression is absent in the anterior-dorsal part of the lateral wall. There is some expression in the posterior-ventral part.
|
Specimen
S2D:
postnatal day 0
(more )
Note: Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
Specimen
S2D:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: telencephalon |
Present |
Regionally restricted |
S2D''
,
S2D
|
Expression is in the anterior-dorsal part of the lateral wall.
|
Specimen
S2E:
postnatal day 15
(more )
Note: Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
Specimen
S2E:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
S2E''
,
S2E
|
Expression is absent in the anterior-dorsal part of the lateral wall. There is some expression in the posterior-ventral part.
|
Specimen
S2G:
postnatal day 15
(more )
Note: Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
Specimen
S2G:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - Foxj1 (assay MGI:7580573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
S2G
,
S2G''
|
Expression is in the posterior-ventral part of the lateral ventricle wall.
|
Specimen
S2H:
postnatal day 15
(more )
Note: Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
Specimen
S2H:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Mcidas; red - S100b (assay MGI:7580575).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
S2H''
,
S2H
|
Expression is in the posterior-ventral part of the lateral ventricle wall.
|
Specimen
not shown P0:
postnatal day 0
(more )
Specimen
not shown P0:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: telencephalon |
Present |
Regionally restricted |
|
Expression is in the anterior-dorsal part of the lateral wall.
|
Specimen
not shown P6:
postnatal day 6
(more )
Specimen
not shown P6:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
|
Expression is in the anterior-dorsal part of the lateral wall.
|
Specimen
not shown P15:
postnatal day 15
(more )
Specimen
not shown P15:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
|
Expression is in the posterior-ventral part.
|
Specimen
not shown Adult:
postnatal adult
(more )
Specimen
not shown Adult:
(close )
Genetic Background:
|
either: C57BL/6 or Parkes
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
|
Expression is in the posterior-ventral part.
|
|