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Gene Expression Data
Assay Details
Assay
Reference: J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type: RNA in situ
MGI Accession ID: MGI:7576024
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2C: embryonic day 12.5 (more )
Note: Pseudo-overlay: red - Fgf8; green - Nkx2-9 (assay MGI:7576004).
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 2C Fgf8 , 2C merge Coexpressed with Nkx2-9 in the isthmic organizer.
Specimen 7I +/+ lateral to medial 1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I +/+ left Expressed in the isthmic organizer.
Specimen 7I +/+ lateral to medial 2: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I +/+ middle Expressed in the isthmic organizer.
Specimen 7I +/+ lateral to medial 3: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I +/+ right Expressed in the isthmic organizer.
Specimen 7I LacZ/LacZ lateral to medial 1: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I LacZ/LacZ left Expressed in the isthmic organizer, similar as in the wild-type.
Specimen 7I LacZ/LacZ lateral to medial 2: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I LacZ/LacZ middle Expressed in the isthmic organizer, similar as in the wild-type.
Specimen 7I LacZ/LacZ lateral to medial 3: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 7I LacZ/LacZ right Expressed in the isthmic organizer, similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory