Gene Expression Data
Assay Details
Assay
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Reference:
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J:137018
Voss AK, et al., C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment. Development. 2008 Jun;135(12):2139-49
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7572984
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Gene symbol:
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Nes
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Gene name:
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nestin
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Results
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Specimen
4A:
embryonic day 12.5
(more )
Note: Double labeled: green - Nes; red - Laminin.
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 12.5
|
Sex:
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Not Specified
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Type:
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section
|
Note:
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Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4A
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Immunofluorescence, marking radial glial cell processes, was observed along cell processes with ventricular-to-pial orientation, essentially parallel to each other.
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Specimen
4B:
(close )
Genetic Background:
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either: (involves: 129S2/SvPas * CBA) or (involves: 129S2/SvPas * CD-1)
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Mutant Allele(s):
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Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5Pgr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4B
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Immunolabeled radial glial fibres in the C3G<gt/gt> mutants were disorganised, lacked ventricular-to-pial orientation and were entangled. Areas of overlap between laminin and Nes were similar in number in C3G<gt/gt> mutants compared with control at E12.5.
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Specimen
4C:
embryonic day 13.5
(more )
Note: Double labeled: green - Nes; red - Laminin.
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4C
|
Immunofluorescence, marking radial glial cell processes, was observed along cell processes with ventricular-to-pial orientation, essentially parallel to each other.
|
Specimen
4D:
(close )
Genetic Background:
|
either: (involves: 129S2/SvPas * CBA) or (involves: 129S2/SvPas * CD-1)
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Mutant Allele(s):
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Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5Pgr
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4D
|
Immunolabeled radial glial fibres in the C3G<gt/gt> mutants were disorganised, lacked ventricular-to-pial orientation and were entangled.
|
Specimen
4E:
embryonic day 13.5
(more )
Note: Double labeled: green - Nes; red - Laminin.
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4E
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Numerous small areas of overlap were seen with laminin basement membrane staining, representing anchoring of radial glial fibers.
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Specimen
4F:
(close )
Genetic Background:
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either: (involves: 129S2/SvPas * CBA) or (involves: 129S2/SvPas * CD-1)
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Mutant Allele(s):
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Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5Pgr
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4F
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Areas of overlap between Nes and laminin were significantly reduced in the mutants compared with wild type.
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Specimen
4G:
embryonic day 14.5
(more )
Note: Double labeled: green - Nes; red - Laminin.
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4G
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Numerous small areas of overlap were seen with laminin basement membrane staining, representing anchoring of radial glial fibers.
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Specimen
4H:
(close )
Genetic Background:
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either: (involves: 129S2/SvPas * CBA) or (involves: 129S2/SvPas * CD-1)
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Mutant Allele(s):
|
Rapgef1Gt(pGT1.8geo)5Pgr/Rapgef1Gt(pGT1.8geo)5Pgr
|
Age:
|
embryonic day 14.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: green - Nes; red - Laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex |
forebrain radial glial cell |
Present |
Regionally restricted |
4H
|
Areas of overlap between Nes and laminin were significantly reduced in the mutants compared with wild type.
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