Car3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:263097 Silagi ES, et al., Expression of Carbonic Anhydrase III, a Nucleus Pulposus Phenotypic Marker, is Hypoxia-responsive and Confers Protection from Oxidative Stress-induced Cell Death. Sci Rep. 2018 Mar 20;8(1):4856
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7572870
Gene symbol:	Car3
Gene name:	carbonic anhydrase 3

Antibody:	anti-CAIII
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

1c ©

1c" ©

1c"' ©

1c' ©

Specimen 1c: postnatal month 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS28: nucleus pulposus	Strong	Uniform	1c' , 1c , 1c"	Expression was homogenous in each cell.
TS28: annulus fibrosus	Absent		1c" , 1c
TS28: vertebra	Absent		1c , 1c"'	No expression in either the endplate or the growth-plate.

1d ©

1d' ©

Specimen 1d: postnatal month 12.5 (more

)
Note: Double labeled: red - Car3; green - Krt19 (assay MGI:7572894).

Structure	Level	Pattern	Image	Note
TS28: nucleus pulposus	Strong		1d' , 1d	Coexpressed with Krt19.

1e ©

1e' ©

Specimen 1e: postnatal month 12.5 (more

)
Note: Double labeled: red - Car3; green - Car12 (assay MGI:7572893).

Structure	Level	Pattern	Image	Note
TS28: nucleus pulposus	Strong		1e' , 1e	Coexpressed with Car12.

3b ©

3d ©

Specimen 3b/d: embryonic day 15.5; Hif1a^tm3Rsjo/Hif1a^tm3Rsjo (more

)

Structure	Level	Pattern	Image	Note
TS23: nucleus pulposus	Present		3d , 3b
TS23: annulus fibrosus	Absent		3b , 3d

3c ©

3e ©

Specimen 3c/e: embryonic day 15.5; Foxa2^tm1(cre)Heli/Foxa2⁺, Hif1a^tm3Rsjo/Hif1a^tm3Rsjo (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: nucleus pulposus	Present		3c , 3e	Similar as in the control.
TS23: annulus fibrosus	Absent		3c , 3e

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24