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Gene Expression Data
Assay Details
Assay
Reference: J:308476 Le Rolle M, et al., Arrest of WNT/beta-catenin signaling enables the transition from pluripotent to differentiated germ cells in mouse ovaries. Proc Natl Acad Sci U S A. 2021 Jul 27;118(30):e2023376118
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7572817
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Results
Specimen 5A 12.5 dpc: embryonic day 12.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS20: germ cell of ovary Present Regionally restricted 5A 12.5 dpc POU5F1 , 5A 12.5 dpc MERGE Expression in nucleus of gonocytes.
Specimen 5A 14.5 dpc: embryonic day 14.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present Regionally restricted 5A 14.5 dpc MERGE , 5A 14.5 dpc POU5F1 Expression mostly localized to cytoplasm.
Specimen 5B 12.5 dpc: embryonic day 12.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS20: germ cell of ovary Present Regionally restricted 5B 12.5 dpc POU5F1 , 5B 12.5 dpc MERGE Expression in nucleus of gonocytes.
Specimen 5B 14.5 dpc: embryonic day 14.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present Regionally restricted 5B 14.5 dpc POU5F1 , 5B 14.5 dpc MERGE Expression mostly localized to cytoplasm.
Specimen 7F Control: embryonic day 13.5 (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7572826).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 7F upper Control inset , 7F upper Control
Specimen 7F Gsk3b-/-: embryonic day 13.5; Gsk3btm1Jrw/Gsk3btm1Jrw (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7572826).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present Regionally restricted 7F upper Gsk3b-/- inset , 7F upper Gsk3b-/- Increase in percentage of expressing germ cells.
Specimen S7C Control: embryonic day 13.5 (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7572826).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present S7C upper Control MERGE , S7C upper Control POU5F1
Specimen S7C Gsk3b-/-: embryonic day 13.5; Gsk3btm1Jrw/Gsk3btm1Jrw (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7572826).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present Regionally restricted S7C upper Gsk3b-/- MERGE , S7C upper Gsk3b-/- POU5F1 Increase in percentage of expressing germ cells.
Specimen 8D XX Control: embryonic day 13.5 (more )
Note: Double labeled: red - Pou5f1; green - Sycp3 (assay MGI:7572831).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 8D XX Control merged , 8D XX Control POU5F1 , 8D XX Control inset
Specimen 8D XX Znrf3-/-: embryonic day 13.5; Znrf3tm1Cle/Znrf3tm1Cle (more )
Note: Double labeled: red - Pou5f1; green - Sycp3 (assay MGI:7572831).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present Regionally restricted 8D XX Znrf3-/- inset , 8D XX Znrf3-/- merged , 8D XX Znrf3-/- POU5F1 Increase in percentage of expressing germ cells.
Specimen S8B 11.5 dpc: embryonic day 11.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present S8B 11.5 dpc MERGE , S8B 11.5 dpc POU5F1
Specimen S8B 12.5 dpc: embryonic day 12.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS20: germ cell of ovary Present S8B 12.5 dpc POU5F1 , S8B 12.5 dpc MERGE
Specimen S8B 13.5 dpc: embryonic day 13.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS21: germ cell of ovary Present S8B 13.5 dpc MERGE , S8B 13.5 dpc POU5F1
Specimen S8B 14.5 dpc: embryonic day 14.5 (more )
Note: Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7572819).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present S8B 14.5 dpc POU5F1 , S8B 14.5 dpc MERGE
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory