Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:122792
Jacobs FM, et al., Retinoic acid counteracts developmental defects in the substantia nigra caused by Pitx3 deficiency. Development. 2007 Jul;134(14):2673-84
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7571202
|
|
Gene symbol:
|
Aldh1a1
|
|
Gene name:
|
aldehyde dehydrogenase family 1, subfamily A1
|
|
Antibody:
|
anti-Aldh1a1
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 555
|
|
|
Results
|
Specimen
1CB:
postnatal adult
(more  )
Note: Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
Specimen
1CB:
(close  )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: substantia nigra pars compacta |
dopaminergic neuron |
Present |
Regionally restricted |
1CC
,
1CH
,
1CI
,
1CG
,
1CB
|
-expression with Th protein in subpopulation of meso-diencephalic (mdDA) neurons located in substantia nigra pars compacta and ventral tegmental nucleus. All expressing cells co-expressed Th.
|
Specimen
1CE:
postnatal adult
(more )
Note: Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
Specimen
1CE:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: ventral tegmental area |
dopaminergic neuron |
Present |
Regionally restricted |
1CL
,
1CK
,
1CE
,
1CF
,
1CJ
|
-expression with Th protein in subpopulation of meso-diencephalic (mdDA) neurons located in substantia nigra pars compacta and ventral tegmental nucleus. All expressing cells co-expressed Th.
|
Specimen
S1C/E:
postnatal adult
(more )
Specimen
S1C/E:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S1D/F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
|
Mutant Allele(s):
|
Pitx3ak/Pitx3ak
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
7BB:
embryonic day 14.5
(more )
Note: Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
Specimen
7BB:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Present |
Regionally restricted |
7BC
,
7BB
|
Co-expression with Th protein in specific subpopulation of meso-diencephalic dopaminergic (mdDA) neurons located in rostro-lateral positions and defining developing substantia nigra pars compacta.
|
Specimen
7BE:
embryonic day 14.5; Pitx3ak/ Pitx3ak
(more )
Note: Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
Specimen
7BE:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
|
Mutant Allele(s):
|
Pitx3ak/Pitx3ak
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Aldh1a1; green - Th (assay MGI:7571201).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Present |
Regionally restricted |
7BF
,
7BE
|
Expression not detected in caudal section of meso-diencephalic dopaminergic (mdDA) area where ventral tegmental area is formed.
|
|
Analysis Tools