Gene Expression Data
Assay Details
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Assay
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Reference:
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J:122792
Jacobs FM, et al., Retinoic acid counteracts developmental defects in the substantia nigra caused by Pitx3 deficiency. Development. 2007 Jul;134(14):2673-84
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7571200
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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Anti-Tyrosine hydroxylase
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1BA,BB,BC:
postnatal adult
(more  )
Note: Double labeled: brown - Th; purple - Aldh1a1 (assay MGI:7571192).
Specimen
1BA,BB,BC:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Th; purple - Aldh1a1 (assay MGI:7571192).
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Specimen
1BD,BE,BF:
postnatal adult
(more )
Note: Double labeled: brown - Th; purple - Aldh1a1 (assay MGI:7571192).
Specimen
1BD,BE,BF:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Th; purple - Aldh1a1 (assay MGI:7571192).
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Specimen
7CA:
embryonic day 14.5
(more )
Specimen
7CA:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Present |
Regionally restricted |
7CA
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Expression in neurons in meso-diencephalic dopaminergic (mdDA) area.
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Specimen
7CC:
embryonic day 14.5
(more )
Specimen
7CC:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Present |
Regionally restricted |
7CC
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Expression in neurons in meso-diencephalic dopaminergic (mdDA) area.
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Specimen
7CE:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Pitx3ak/Pitx3ak
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Weak |
Regionally restricted |
7CE
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Significant loss of expressing neurons in ventro-lateral regions.
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Specimen
7CG:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Pitx3ak/Pitx3ak
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
dopaminergic neuron |
Present |
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7CG
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Specimen
8AA:
embryonic day 18.5
(more )
Specimen
8AA:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: substantia nigra |
Present |
Regionally restricted |
8AA
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Expression at level of substantial nigra pars compacta.
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Specimen
8AB:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Pitx3ak/Pitx3ak
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: substantia nigra |
Present |
Regionally restricted |
8AB
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Expression at level of substantial nigra pars compacta.
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Specimen
8DA:
embryonic day 18.5
(more )
Specimen
8DA:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8DC:
embryonic day 18.5
(more )
Specimen
8DC:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8DE:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Pitx3ak/Pitx3ak
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8DG:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Pitx3ak/Pitx3ak
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools