Trp53 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283328 Klimovich B, et al., Inactivation of Mdm2 restores apoptosis proficiency of cooperativity mutant p53 in vivo. Cell Cycle. 2020 Jan;19(1):109-123
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7569304
Gene symbol:	Trp53
Gene name:	transformation related protein 53

Antibody:	anti-p53 (CM5) (NCL-p53-505)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 3a Mdm2+/-: embryonic day 10.5; Mdm2^tm1.1Mep/Mdm2⁺, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Image
TS17: embryo	Absent	3a Mdm2+/-;Trp53RR/RR
TS17: brain	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (1)
TS17: heart	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (2)
TS17: future spinal cord	Absent	3a +/- (3) , 3a Mdm2+/-;Trp53RR/RR
TS17: somite	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (4)
TS17: limb bud	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (5)
TS17: embryo mesenchyme	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (6)

Specimen 3a Mdm2-/-: embryonic day 10.5; Mdm2^tm1.1Mep/Mdm2^tm1.1Mep, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

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Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	3a Mdm2-/-;Trp53RR/RR	Strong accumulation of expression in all tissues except heart.
TS17: brain	Strong		3a Mdm2-/-;Trp53RR/RR , 3a -/- (1)	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: heart	Weak		3a -/- (2) , 3a Mdm2-/-;Trp53RR/RR	Markedly lower expression in cardiac tissues.
TS17: future spinal cord	Strong		3a Mdm2-/-;Trp53RR/RR , 3a -/- (3)	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: somite	Strong		3a -/- (4) , 3a Mdm2-/-;Trp53RR/RR	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: limb bud	Strong		3a -/- (5) , 3a Mdm2-/-;Trp53RR/RR	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: embryo mesenchyme	Present		3a -/- (6) , 3a Mdm2-/-;Trp53RR/RR

Specimen 4e Mdm2+/-: embryonic day 10.5; Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj, Mdm2^tm1.1Mep/Mdm2⁺, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Image
TS17: embryo	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: brain	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/- , 4e +/- (1)
TS17: heart	Absent	4e +/- (2) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: future spinal cord	Absent	4e +/- (3) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: somite	Absent	4e +/- (4) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: limb bud	Absent	4e +/- (5) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: embryo mesenchyme	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/- , 4e +/- (6)

Specimen 4e Mdm2-/-: embryonic day 10.5; Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj, Mdm2^tm1.1Mep/Mdm2^tm1.1Mep, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-	Authors report strong accumulation of expression.
TS17: brain	Strong		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (1)
TS17: heart	Present		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (2)
TS17: future spinal cord	Strong		4e -/- (3) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-
TS17: somite	Strong		4e -/- (4) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-
TS17: limb bud	Strong		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (5)
TS17: embryo mesenchyme	Strong		4e -/- (6) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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