Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171473
Nie X, et al., Six1 regulates Grem1 expression in the metanephric mesenchyme to initiate branching morphogenesis. Dev Biol. 2011 Apr 1;352(1):141-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7567720
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Gene symbol:
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Ret
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Gene name:
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ret proto-oncogene
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Probe:
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C-ret probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: ureteric bud |
Present |
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1A
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Normal outgrowth and the formation of tip domain were seen.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129/SvEv * 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: ureteric bud |
Present |
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1B
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Outgrowth, its subdivision into the trunk and tip domains and elongation appeared to occur normally.
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Specimen
1C:
embryonic day 11.0
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1D:
(close )
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Genetic Background:
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involves: 129/SvEv * 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: ureteric tip |
Present |
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1D
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Reduced expression compared with the wild-type. The tip region was not dilated for the formation of ampulla.
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| TS18: ureteric stalk |
Present |
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1D
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Similar as in the wild-type.
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129/SvEv * 129S2/SvPas
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: ureteric tip |
Present |
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1F
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Reduced expression compared with the wild-type. The tip region was not dilated for the formation of ampulla.
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| TS19: ureteric stalk |
Present |
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1F
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Similar as in the wild-type.
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Analysis Tools