Gene Expression Data
Assay Details
Assay
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Reference:
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J:236076
Gai M, et al., ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules. EMBO Rep. 2016 Oct;17(10):1396-1409
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7562402
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Results
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Specimen
1C WT:
embryonic day 14.5
(more )
Note: Triple labeled: red - Mki67; blue - Eomes (assay MGI:7562404); green - BrdU.
Specimen
1C WT:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Mki67; blue - Eomes (assay MGI:7562404); green - BrdU.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: neocortex |
Present |
Non-Uniform |
1C WT
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Specimen
1C CITK-/-:
embryonic day 14.5; Cittm1Fdc/ Cittm1Fdc
(more )
Note: Triple labeled: red - Mki67; blue - Eomes (assay MGI:7562404); green - BrdU.
Specimen
1C CITK-/-:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Cittm1Fdc/Cittm1Fdc
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Mki67; blue - Eomes (assay MGI:7562404); green - BrdU.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex intermediate zone |
Present |
Non-Uniform |
1C CITK-/- boxed area
,
1C CITK-/-
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A significant increase in BrdU-positive/Mki67-negative cells was observed in the proliferative regions and in the intermediate zone of the mutant.
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TS22: neocortex |
Present |
Non-Uniform |
1C CITK-/- boxed area
,
1C CITK-/-
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A significant increase in BrdU-positive/Mki67-negative cells was observed in the proliferative regions and in the intermediate zone of the mutant.
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