Gene Expression Data
Assay Details
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Assay
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Reference:
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J:175963
Breau MA, et al., A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme. Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7750-5
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7548593
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Gene symbol:
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Cdh1
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Gene name:
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cadherin 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
5A:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more  )
Note: 4-5 somites. Fix: 1% PFA.
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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4-5 somites. Fix: 1% PFA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural fold |
Present |
Regionally restricted |
5A
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Expression was detected in domains of the cranial neural folds that are distinct from the neuroepithelium.
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Specimen
5B:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA.
Specimen
5B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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4-5 somites. Fix: 1% PFA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural fold |
Present |
Regionally restricted |
5B
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Expression was detected in domains of the cranial neural folds that are distinct from the neuroepithelium.
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Specimen
5C:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA.
Specimen
5C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4-5 somites. Fix: 1% PFA.
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Specimen
5E:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA.
Specimen
5E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4-5 somites. Fix: 1% PFA.
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Specimen
5G:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA. Double labeled: blue - Cdh1 reporter; brown - Pdgfra (assay MGI:7548548).
Specimen
5G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4-5 somites. Fix: 1% PFA. Double labeled: blue - Cdh1 reporter; brown - Pdgfra (assay MGI:7548548).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS12: head |
epithelial cell |
Present |
Regionally restricted |
5G inset
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5H
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5G
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Expression was detected in non-neural epithelial cells of the head. Coexpression with Pdgfra was observed.
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| TS12: head mesenchyme |
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Present |
Regionally restricted |
5G
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5G inset
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5H
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Expression was detected in mesenchyme underlying the non-neural epithelial cells. Coexpression with Pdgfra was observed.
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Specimen
5I:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA. Double labeled: blue - Cdh1 reporter; brown - Pdgfra (assay MGI:7548548).
Specimen
5I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4-5 somites. Fix: 1% PFA. Double labeled: blue - Cdh1 reporter; brown - Pdgfra (assay MGI:7548548).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS12: head |
epithelial cell |
Present |
Regionally restricted |
5J
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5I inset
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5I
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Expression was detected in non-neural epithelial cells of the head. Coexpression with Pdgfra was observed.
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| TS12: head mesenchyme |
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Present |
Regionally restricted |
5I inset
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5I
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5J
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Expression was detected in mesenchyme underlying the non-neural epithelial cells. Coexpression with Pdgfra was observed.
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Specimen
not shown:
embryonic day 8.5; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: Fix: 1% PFA.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Fix: 1% PFA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Single cells |
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A few labeled cells were detected within the neural epithelium.
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Specimen
S2A:
embryonic day 8.0; Cdh1tm3.1Kem/Cdh1 +
(more )
Note: 4-5 somites. Fix: 1% PFA.
Specimen
S2A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Cdh1tm3.1Kem/Cdh1+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4-5 somites. Fix: 1% PFA.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural fold |
Present |
Regionally restricted |
S2B
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S2A
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Expression was detected in nonneural epithelium of rostral neural fold.
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| TS12: head mesenchyme |
Present |
Regionally restricted |
S2B
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S2A
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Expression was detected in the mesenchyme underlying nonneural epithelium of rostral neural fold.
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