H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:342123 Sterling NA, et al., P53 independent pathogenic mechanisms contribute to BubR1 microcephaly. Front Cell Dev Biol. 2023;11:1282182
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7548343
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-gammaH2AX (Ser139) (05-636)
Detection system:	Secondary antibody

Results

1D WT (left) ©

Specimen 1D WT: embryonic day 14.5 (more

)
Note: Double labeled: red - H2ax; red - phospho-histone H3.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Absent		1D WT (middle) , 1D WT (left) , 1D WT (right)

Specimen 1D BubR1 cKO: embryonic day 14.5; Bub1b^tm1Jvd/Bub1b^tm1Jvd, Emx1^tm1(cre)Krj/Emx1⁺ (more

)
Note: Conditional mutant. Double labeled: red - H2ax; red - phospho-histone H3.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		1D BubR1 cKO (right) , 1D BubR1 cKO (middle) , 1D BubR1 cKO (left)	Significant increase in expression compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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