Cspg4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:110095 Ligon KL, et al., Development of NG2 neural progenitor cells requires Olig gene function. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7853-8
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7547381
Gene symbol:	Cspg4
Gene name:	chondroitin sulfate proteoglycan 4

Antibody:	anti-NG2 (AB5320)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

3A +/- ©

Specimen 3A +/-: embryonic day 14.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex	pericyte	Present		3A +/-
TS22: cerebral cortex		Present	Scattered	3A +/-	Expressed in neural progenitor cells with synantocyte or bipolar morphology.

3A -/- ©

Specimen 3A -/-: embryonic day 14.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex	pericyte	Present	Regionally restricted	3A -/-	Only staining in vessel pericytes was unaffected. No scattered positive neural progenitor cells with synantocyte or bipolar morphology were seen.

3B +/- 10X ©

Specimen 3B +/- 10x: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: brain		Present		3B +/- 10X	Expressed in multiprocessed cells.
TS26: brain	pericyte	Present	Regionally restricted	3B +/- 10X	Expressed in in vessel pericytes.

3B -/- 10X ©

Specimen 3B -/- 10x: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: brain	pericyte	Present	Regionally restricted	3B -/- 10X	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

3B +/- CX ©

Specimen 3B +/- CX: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: neocortex		Present		3B +/- CX	Expressed in multiprocessed cells.
TS26: neocortex	pericyte	Present	Regionally restricted	3B +/- CX	Expressed in in vessel pericytes.

3B -/- CX ©

Specimen 3B -/- CX: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: neocortex	pericyte	Present	Regionally restricted	3B -/- CX	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

3B +/- CC ©

Specimen 3B +/- CC: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: corpus callosum		Present		3B +/- CC	Expressed in multiprocessed cells.
TS26: corpus callosum	pericyte	Present	Regionally restricted	3B +/- CC	Expressed in in vessel pericytes.

3B -/- CC ©

Specimen 3B -/- CC: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: corpus callosum	pericyte	Present	Regionally restricted	3B -/- CC	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

3B +/- SVZ ©

Specimen 3B +/- SVZ: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone		Present		3B +/- SVZ	Expressed in multiprocessed cells.
TS26: cerebral cortex subventricular zone	pericyte	Present	Regionally restricted	3B +/- SVZ	Expressed in in vessel pericytes.

3B -/- SVZ ©

Specimen 3B -/- SVZ: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	pericyte	Present	Regionally restricted	3B -/- SVZ	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

3B +/- OB ©

Specimen 3B +/- OB: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: main olfactory bulb		Present		3B +/- OB	Expressed in multiprocessed cells.
TS26: main olfactory bulb	pericyte	Present	Regionally restricted	3B +/- OB	Expressed in in vessel pericytes.

3B -/- OB ©

Specimen 3B -/- OB: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: main olfactory bulb	pericyte	Present	Regionally restricted	3B -/- OB	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

3B +/- VFB 10X ©

3B +/- VFB 40X ©

Specimen 3B +/- VFB: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: anterior commissure		Present		3B +/- VFB 40X , 3B +/- VFB 10X	Expressed in multiprocessed cells within the ventral forebrain in the region of the anterior commissure.
TS26: anterior commissure	pericyte	Present	Regionally restricted	3B +/- VFB 40X , 3B +/- VFB 10X	Expressed in in vessel pericytes.

Specimen 3B -/- VFB: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: anterior commissure	pericyte	Present	Regionally restricted	3B -/- VFB 10X , 3B -/- VFB 40X	Staining in vessel pericytes was unaffected.
TS26: anterior commissure		Present	Scattered	3B -/- VFB 40X , 3B -/- VFB 10X	Scattered surviving cells were detected within the ventral forebrain in the region of the anterior commissure.

3C left ©

Specimen 3C Olig2-/-: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: forebrain		Present	Scattered	3C left	Scattered surviving cells were detected in the ventral forebrain.
TS26: forebrain	pericyte	Present	Regionally restricted	3C left	Staining in vessel pericytes was unaffected.

3C right ©

Specimen 3C Olig1/2-/-: embryonic day 18.5; Olig1^tm1And/Olig1^tm1And, Olig2^tm1And/Olig2^tm1And (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: forebrain	pericyte	Present	Regionally restricted	3C right	Only staining in vessel pericytes was unaffected. No positive cell bodies with recognizable synantocyte or bipolar morphology were seen.

4A lower +/- ©

Specimen 4A +/-: embryonic day 18.5; Olig2^tm1Rth/Olig2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord		Present		4A middle +/- , 4A lower +/-	Expressed in multiprocessed cells.
TS26: spinal cord	pericyte	Present	Regionally restricted	4A middle +/- , 4A lower +/-	Expressed in in vessel pericytes.

4A lower -/- ©

Specimen 4A -/-: embryonic day 18.5; Olig2^tm1Rth/Olig2^tm1Rth (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: spinal cord	pericyte	Present	Regionally restricted	4A middle -/- , 4A lower -/-	Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.

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