Gene Expression Data
Assay Details
Assay
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Reference:
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J:311540
Oliver BL, et al., Spatial and temporal expression of PORCN is highly dynamic in the developing mouse cochlea. Gene Expr Patterns. 2021 Sep 20;42:119214
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7547375
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Gene symbol:
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Wls
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Gene name:
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wntless WNT ligand secretion mediator
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Probe:
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Mm-Wls (405011)
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Probe preparation:
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Antisense
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Visualized with:
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Chromogenic
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Assay notes:
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RNAscope assay.
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Results
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Specimen
3A:
embryonic day 12.5
(more )
Note: Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Specimen
3Bi:
embryonic day 14.5
(more )
Note: base t1. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Bi:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
base t1. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlear duct |
Present |
Widespread |
3Bi
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Expression is seen across the radial and longitudinal axes.
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Specimen
3Bii:
embryonic day 14.5
(more )
Note: mid t2. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Bii:
(close )
Genetic Background:
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Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
mid t2. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlear duct |
Present |
Non-Uniform |
3Bii
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Expression is slightly enriched in the lateral half of the cochlea.
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Specimen
3Biii:
embryonic day 14.5
(more )
Note: apex t3. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Biii:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
apex t3. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlear duct |
Present |
Non-Uniform |
3Biii
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Expression is slightly enriched in the lateral half of the cochlea.
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Specimen
3Ci:
embryonic day 16.5
(more )
Note: base t1. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Ci:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
base t1. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct |
Present |
Non-Uniform |
3Ci
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Expression is downregulated in the sensory domain.
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Specimen
3Cii:
embryonic day 16.5
(more )
Note: mid t2. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Cii:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
mid t2. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct |
Present |
Non-Uniform |
3Cii
|
Expression is downregulated in the sensory domain.
|
Specimen
3Ciii:
embryonic day 16.5
(more )
Note: mid t3. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Ciii:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
mid t3. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct |
Present |
Non-Uniform |
3Ciii
|
Expression is downregulated in the sensory domain.
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Specimen
3Civ:
embryonic day 16.5
(more )
Note: apex t4. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
Specimen
3Civ:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
apex t4. Double labeled: green - Wls; red - Porcn (assay MGI:7547379).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct |
Present |
Non-Uniform |
3Civ
|
Expression is downregulated in the sensory domain.
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