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Gene Expression Data
Assay Details
Assay
Reference: J:273130 Ding W, et al., Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex. J Neurosci. 2019 Mar 13;39(11):1994-2010
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7547347
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-GFAP (G9269)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 5E GFAP Cenpj+/+ (left): postnatal adult (more )
Structure Level Pattern Image Note
TS28: telencephalon Present Regionally restricted 5E Cenpj+/+ GFAP (left lower) , 5E Cenpj+/+ GFAP (left upper) Expressing cells highly enriched on surface of lateral ventricle.
Specimen 5E GFAP CenpjCKO (left): postnatal adult; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: telencephalon Trace Regionally restricted 5E CenpjCKO GFAP (left upper) , 5E CenpjCKO GFAP (left lower) Only a few expressing cells were observed.
Specimen 5E GFAP Cenpj+/+ (right): postnatal adult (more )
Note: Double labeled: green - Gfap; red - Mki67 (assay MGI:7547348).
Structure Level Pattern Image Note
TS28: telencephalon Present Regionally restricted 5E Cenpj+/+ (right upper) , 5E Cenpj+/+ (right middle) Expressing cells highly enriched on surface of lateral ventricle.
Specimen 5E GFAP CenpjCKO (right): postnatal adult; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Gfap; red - Mki67 (assay MGI:7547348).
Structure Level Pattern Image Note
TS28: telencephalon Trace Regionally restricted 5E CenpjCKO (right upper) , 5E CenpjCKO (right middle) Only a few expressing cells were observed
Specimen 5H GFAP Cenpj+/+CiliaGFP/+: postnatal day 40; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: gray - Gfap; red - Ctnnb1 (assay MGI:7547349); green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS28: brain subventricular zone neural stem cell Present Regionally restricted 5H Cenpj+/+ GFAP , 5H Cenpj+/+ (merged right) , 5H Cenpj+/+ (merged left) Expression in B1 neural stem cells.
TS28: brain ventricular layer neural stem cell Present Regionally restricted 5H Cenpj+/+ GFAP , 5H Cenpj+/+ (merged right) , 5H Cenpj+/+ (merged left) Expression in B1 neural stem cells.
Specimen 5H GFAP CenpjCKOCiliaGFP/+: postnatal day 40; Cpaptm1c(EUCOMM)Wtsi/Cpaptm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Gfap; red - Ctnnb1 (assay MGI:7547349); green - GFP reporter.
Structure Level Pattern Image Note
TS28: brain subventricular zone Trace 5H CenpjCKO (merged left) , 5H CenpjCKO (merged right) , 5H CenpjCKO GFAP Expression reduced compared to wild type.
TS28: brain ventricular layer Trace 5H CenpjCKO (merged left) , 5H CenpjCKO (merged right) , 5H CenpjCKO GFAP Expression reduced compared to wild type.
Specimen 5I GFAP Cenpj+/+CiliaGFP/+: postnatal day 40; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Gfap; blue - Ctnnb1 (assay MGI:7547349); green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS28: brain ventricular layer neural stem cell Present Regionally restricted 5I Cenpj+/+ (merged right) , 5I Cenpj+/+ GFAP , 5I Cenpj+/+ (merged left) Expression in B1 neural stem cells.
Specimen 5I GFAP CenpjCKOCiliaGFP/+: postnatal day 40; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Gfap; blue - Ctnnb1 (assay MGI:7547349); green - GFP reporter.
Structure Level Pattern Image Note
TS28: brain subventricular zone Trace 5I CenpjCKO GFAP , 5I CenpjCKO (merged right) , 5I CenpjCKO (merged left) Expression reduced compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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