Gene Expression Data
Assay Details
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Assay
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Reference:
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J:257227
Fujikawa J, et al., Kruppel-like factor 4 regulates matrix metalloproteinase and aggrecanase gene expression in chondrocytes. Cell Tissue Res. 2017 Dec;370(3):441-449
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7543971
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Gene symbol:
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Klf4
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Gene name:
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Kruppel-like transcription factor 4 (gut)
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Antibody:
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anti-KLF4 (H-180)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1a:
embryonic day 14.5
(more  )
Specimen
1a:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
1b:
embryonic day 15.5
(more )
Specimen
1b:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: forelimb long bone |
osteoclast |
Strong |
Single cells |
1b
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Expression is in large cells at the primary ossification center (presumably osteoclasts).
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| TS23: forelimb cartilage condensation |
chondrocyte |
Present |
Regionally restricted |
1b
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Expression is in immature chondrocytes and is absent in maturing chondrocytes.
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Specimen
1c:
embryonic day 16.5
(more )
Specimen
1c:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forelimb cartilage condensation |
chondrocyte |
Present |
Regionally restricted |
1c
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Expression is in articular chondrocytes and in resting and proliferating zone chondrocytes.
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Specimen
1d:
embryonic day 16.5
(more )
Specimen
1d:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forelimb long bone |
osteoclast |
Strong |
Single cells |
1d
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Expression is in large cells at the primary ossification center (presumably osteoclasts).
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| TS24: forelimb cartilage condensation |
chondrocyte |
Present |
Regionally restricted |
1d
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Expression is in articular chondrocytes and in resting and proliferating zone chondrocytes.
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Specimen
1e:
postnatal day 6
(more )
Specimen
1e:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: forearm bone |
chondrocyte |
Present |
Regionally restricted |
1e
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Expression is in articular chondrocytes and in immature chondrocytes. Absent from the region forming the secondary ossification center.
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Specimen
1f:
postnatal day 6
(more )
Specimen
1f:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: forearm bone |
chondrocyte |
Strong |
Regionally restricted |
1f
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Expression is in prehypertrophic and hypertrophic chondrocytes in the the growth plate.
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| TS28: forearm bone |
hypertrophic chondrocyte |
Strong |
Regionally restricted |
1f
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Expression is in prehypertrophic and hypertrophic chondrocytes in the the growth plate.
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Specimen
2a:
postnatal month 6
(more )
Specimen
2a:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal month 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
articular chondrocyte |
Present |
Regionally restricted |
2e
,
2c
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2a
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Expression is at the articular surface and is weak in the articular chondrocytes.
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| TS28: femur |
articular chondrocyte |
Present |
Regionally restricted |
2e
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2c
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2a
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Expression is at the articular surface and is weak in the articular chondrocytes.
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| TS28: tibia |
articular chondrocyte |
Present |
Regionally restricted |
2e
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2c
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2a
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Expression is at the articular surface and is weak in the articular chondrocytes.
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