Antibody:	anti-GAD65/67 (AB1511)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spinal cord gray matter		Present		1A
TS28: spinal cord dorsal horn	GABAergic neuron	Present	Regionally restricted	1A	Expression in GABAergic terminals, detected as dots of expression, numerous in dorsal horn.
TS28: spinal cord ventral horn	GABAergic neuron	Weak	Regionally restricted	1A	Few dots of expression in ventral horn.
TS28: spinal cord white matter		Absent		1A	No expression in axon bundles in anterior, lateral and posterior funiculi.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spinal cord ventral horn	GABAergic neuron	Weak	Regionally restricted	1B	Few dots of expression in ventral horn.
TS28: spinal cord white matter		Absent		1B	No expression in ascending and descending axons in white matter.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Absent		2A	No expression in spinal cord, including monolayer of neurons on surface of spinal cord.

Structure	Cell Type	Level	Pattern	Image	Note
TS18: future spinal cord	GABAergic neuron	Present	Regionally restricted	2B	Expression in 3 GABAergic neuron groups: dorsal (G1), middle (G2, largest) and ventral (G3).
TS18: neural tube marginal layer		Present	Regionally restricted	2B	Expression in anterior part of marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS18: neural tube mantle layer		Present		2C	Expression in many neurons localized in mantle layer.
TS18: future spinal cord	GABAergic neuron	Present	Regionally restricted	2C	Expression in middle (G2) group of neuron sending commissural neurons crossing in front of floor plate to contralateral marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS18: neural tube mantle layer	GABAergic neuron	Present	Regionally restricted	2E	Expression in ventral (G3) group of neurons in mantle layer sending axons into axons into ipsilateral marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS18: neural tube marginal layer	GABAergic neuron	Present	Regionally restricted	2F	Expression in ventral (G3) group of neurons in mantle layer sending axons into axons into ipsilateral marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord	GABAergic neuron	Present	Regionally restricted	3A	Expression in 4 GABAergic neuron groups.
TS20: spinal cord mantle layer		Present	Regionally restricted	3A	Expression in G1 neurons localized at dorsal end of mantle layer and on surface of ventricular zone.
TS20: spinal cord ventral horn		Present	Regionally restricted	3A	Expression in G3 neurons forming a neuronal mass in ventral horn.
TS20: spinal cord marginal layer		Present	Regionally restricted	3A	Expression in anterior part of marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord		Present	Regionally restricted	3B	GABA neurons almost absent of surface of ventricular zone. Decreased number of commissural axons.
TS21: spinal cord dorsal horn		Present	Scattered	3B	Expression scattered within ventral part of dorsal horn.
TS21: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	3B	Expression in G3 neurons forming a neuronal mass in ventral horn. Expression in G4 neurons forming a neuronal mass at dorsal position to G3 in ventral horn.
TS21: spinal cord white matter		Strong	Regionally restricted	3B	Increased density of expression in white matter, including anterior, lateral and posterior funiculi.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: spinal cord dorsal horn		Present	Widespread	3C	Expression distributed throughout dorsal horn.
TS23: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	3C	Expression in G3 and G4 neurons intermingled in ventral horn.
TS23: spinal cord white matter		Present	Regionally restricted	3C	Decreased intensity of expression in anteiror and lateral funiculi. Heavy expression in posterior funiculus.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: spinal cord dorsal horn		Strong		3D	Expression in dorsal horn markedly increased in intensity and density.
TS25: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	3D	Expression in G3 and G4 neurons with axons exhibitling a mesh-like structures.
TS25: spinal cord white matter		Present	Regionally restricted	3D	Decreased intensity of expression in anteiror and lateral funiculi. Heavy expression in posterior funiculus.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	4B	Expression in GABAergic neurons, including cell bodies, dendrites and axons.
TS20: spinal cord marginal layer		Present	Regionally restricted	4B	Expression in axons often entering ipsilateral marginal zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	4D	Expression in increased number of GABAergic neurons and axons.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	4F	Expression in GABAergic neurons forming sparce plexus-like structure within gray matter. Varicosities were clearly and diffusely discernable.
TS23: spinal cord white matter		Present	Regionally restricted	4F	Expression in axons often entering ipsilateral white matter.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: spinal cord ventral horn	GABAergic neuron	Present	Regionally restricted	4H	Expression in GABAergic neurons confined to varicosities markedly increased in number and density, and often surrounding cell bodies of large motor neurons.
TS25: spinal cord white matter		Trace	Regionally restricted	4H	Expression almost disappeared from axons.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: spinal cord dorsal horn	GABAergic neuron	Present		5B	Expression in GABAergic neurons first observed on E13.
TS21: spinal cord white matter		Trace	Regionally restricted	5B	Expression in only a few axons entering posterior funiculus.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: spinal cord dorsal horn	GABAergic neuron	Present	Regionally restricted	5D	Expression in increased number of GABAergic neurons and axons.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: spinal cord dorsal horn	GABAergic neuron	Present	Regionally restricted	5F	Expression in density number of GABAergic neurons and axons. Expression mainly confined to varicosities, but still detected between varicosities.