Antibody:	anti-KCC2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Present	Widespread	1E	Expression throughout gray matter.
TS28: spinal cord white matter	Absent		1E	No expression in axon bundles in anterior, lateral and posterior funiculi.

Structure	Level	Pattern	Image	Note
TS28: spinal cord ventral horn	Present		1F

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	8A	Expression first detected on ventral surface of spinal cord.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	8B	Expression slightly or moderately localized within ventral horn.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	8C	Expression gradually increased in intensity, gradually spreading to dorsal part.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	8D	Expression gradually increased in intensity, gradually spreading to dorsal part.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	8E	Expression gradually increased in intensity, gradually spreading to dorsal part.

Structure	Level	Pattern	Image	Note
TS25: spinal cord gray matter	Present	Uniform	8F	Homogeneous expression in gray matter.
TS25: spinal cord ventricular layer	Absent		8F	No expression in white matter and ventricular zone.
TS25: spinal cord white matter	Absent		8F	No expression in white matter and ventricular zone.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Weak	Regionally restricted	8G	Weak expression localized within perikarya of monolayer neurons on surface of spinal cord.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	8H	Moderate or dense expression in a few neurons beneath marginal zone. Weak expression in neuropil of ventral horn.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventral horn	Moderate	Regionally restricted	8I	Moderate expression diffusely detected in neuropil of ventral horn.
TS20: spinal cord marginal layer	Present	Regionally restricted	8I	Expression in dendrites extending into marginal zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventral horn	Strong	Regionally restricted	8J	Increased intensity of expression. Expression in dendrites and cell bodies.

Structure	Level	Pattern	Image	Note
TS23: spinal cord ventral horn	Strong	Regionally restricted	8K	Increased intensity of expression. Expression in dendrites and cell bodies.

Structure	Level	Pattern	Image	Note
TS25: spinal cord ventral horn	Strong	Regionally restricted	8L	Dense expression localized at cell membrane of somata and dendrites.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	8M	Expression in few neurons on surface of dorsal horn.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	8N	Expression in neurons covering surface of dorsal horn.
TS23: spinal cord dorsal horn	Absent		8N

Structure	Cell Type	Level	Pattern	Image	Note
TS25: spinal cord	sensory neuron	Moderate		8O