Antibody:	Anti-Histone H1x (ab31972)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Widespread	2F	Expression was almost ubiquitous. Some immunonegative cells were observed at the caudal area adjacent to the somites including the septum transversum.
TS13: septum transversum	Absent		2F

Structure	Level	Pattern	Image	Note
TS21: hindbrain ventricular layer	Present		4B
TS21: telencephalon ventricular layer	Present		4B

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4E
TS21: inner ear	Present		4E
TS21: retina	Present		4E
TS21: spinal cord ventricular layer	Present		4E

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Present		4H
TS21: vertebral pre-cartilage condensation	Present		4H
TS21: esophagus	Present		4H
TS21: trachea	Present		4H
TS21: heart	Weak		4H

Structure	Level	Pattern	Image	Note
TS21: vertebral pre-cartilage condensation	Present		4K
TS21: gut	Present		4K
TS21: stomach	Present		4K
TS21: liver	Weak		4K

Structure	Cell Type	Level	Pattern	Image	Note
TS21: vertebral pre-cartilage condensation		Present		4N
TS21: liver		Weak		4N
TS21: mesonephric tubule		Present		4Q , 4N
TS21: sympathetic ganglion		Present		4Q , 4N
TS21: adrenal gland		Present		4Q , 4N
TS21: gonad		Present	Regionally restricted	4Q , 4N	Expression was detected in the peripheral zone of the gonad.
TS21: migrating neural crest	neural crest cell	Present	Regionally restricted	4Q , 4N	Immunolabeled neural crest cells in association with the abdominal aorta entered into the adrenal primordium to form the medulla.

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present		5B

Structure	Level	Pattern	Image	Note
TS23: pituitary gland	Present	Regionally restricted	5D	Expression was detected in the posterior part of the pituitary gland.
TS23: hypothalamus	Present	Regionally restricted	5D	Expression was detected in the region of the hypothalamus surrounding the bottom area of the third ventricle.

Structure	Level	Pattern	Image	Note
TS23: sympathetic ganglion	Present		5F
TS23: lobar bronchus	Strong		5F
TS23: lung mesenchyme	Weak		5F

Structure	Level	Pattern	Image	Note
TS23: endocrine pancreas	Present		5I

Structure	Level	Pattern	Image	Note
TS23: adrenal medulla	Present		5L
TS23: early nephron	Weak		5L
TS23: mesenchyme of metanephros	Weak		5L

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Present	Regionally restricted	5N	Expression was detected in enteric neural regions lying outside of the smooth muscle layers. Co-localization was noted with Tubb3.

Structure	Level	Pattern	Image	Note
TS24: olfactory lobe	Present		6B
TS24: telencephalon subventricular zone	Present		6B
TS24: thalamus	Present		6B

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		6G , 6D
TS24: cortical plate	Present		6G , 6D
TS24: cochlea	Present		6D
TS24: hypothalamus	Present		6D
TS24: pineal gland	Present		6D
TS24: 3rd ventricle	Present	Regionally restricted	6D	Expression was detected in the bottom region of the third ventricle.

Structure	Level	Pattern	Image	Note
TS24: retina	Present		6D inset

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	7B	Expression was detected at the inner layers of the olfactory bulb connected to the subventricular zone.
TS27: olfactory lobe	Present		7B
TS27: telencephalon subventricular zone	Present		7B
TS27: hippocampus granule cell layer	Present		7B , 7E

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	7H	Expression was detected at the edge of the subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present		8G , 8B

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Weak	Regionally restricted	8L	Expression was weakly detected in the subgranular zone of the hippocampus.
TS28: cerebral cortex	Weak		8L

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer		Present		8P
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		8P