Gene Expression Data
Assay Details
Assay
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Reference:
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J:69625
Hubner CA, et al., Disruption of KCC2 Reveals an Essential Role of K-Cl Cotransport Already in Early Synaptic Inhibition. Neuron. 2001 May;30(2):515-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7529694
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Gene symbol:
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Slc12a5
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Gene name:
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solute carrier family 12, member 5
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Antibody:
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anti-KCC2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 546
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Results
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Specimen
3B/C:
embryonic day 18.5
(more )
Note: Double labeled: red - Slc12a5; green - Map2 (assay MGI:7529696).
Specimen
3B/C:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 18.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc12a5; green - Map2 (assay MGI:7529696).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
spinal cord motor neuron |
Present |
Regionally restricted |
3B
,
3C
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Coexpression with Map2 in dendrites.
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Specimen
3E/F:
embryonic day 18.5
(more )
Note: Double labeled: red - Slc12a5; green - neurofilament.
Specimen
3E/F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Slc12a5; green - neurofilament.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
spinal cord motor neuron |
Present |
Regionally restricted |
3F
,
3E
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Not coexpressed with neurofilament staining.
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Specimen
3H/I:
postnatal adult
(more )
Note: Double labeled: red - Slc12a5; green - Gphn (assay MGI:7529697).
Specimen
3H/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Slc12a5; green - Gphn (assay MGI:7529697).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: spinal cord motor column |
spinal cord motor neuron |
Present |
Regionally restricted |
3I
,
3H
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Predominantly, but not exclusively, coexpressed in Gphn positive inhibitory synapses.
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Specimen
not shown:
(close )
Genetic Background:
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either: 129X1/SvJ or (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * NMRI)
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Mutant Allele(s):
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Slc12a5tm1Tjj/Slc12a5tm1Tjj
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Age:
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embryonic day 18.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
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