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Gene Expression Data
Assay Details
Assay
Reference: J:68840 Nakagawa Y, et al., Combinatorial expression patterns of LIM-homeodomain and other regulatory genes parcellate developing thalamus. J Neurosci. 2001 Apr 15;21(8):2711-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7524728
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-C-Isl-1 protein (K5)
Assay notes: Antibody also recognizes Isl2, but authors state Isl2 was not expressed in areas studied, thus only Isl1 was detected.
Results
Specimen 7L: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Lhx1 (assay MGI:7524729) and Lhx5 (assay MGI:7524730).
Structure Level Pattern Image Note
TS20: prethalamus Present 7L
Specimen 7M: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Lhx1 (assay MGI:7524729) and Lhx5 (assay MGI:7524730).
Structure Level Pattern Image Note
TS20: prethalamus Present Regionally restricted 7M Isl1-expressing cells rarely overlap with Lhx1/5-expressing cells.
Specimen 7N: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Lhx1 (assay MGI:7524729) and Lhx5 (assay MGI:7524730).
Structure Level Pattern Image Note
TS20: zona incerta Present Regionally restricted 7N Ventral subdivision of zona incerta contains heterogeneous populations of cells for expression of Isl1, Lhx1/5 and Pax6.
Specimen 7O: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Pax6 (assay MGI:7524731).
Structure Level Pattern Image Note
TS20: prethalamus Present Regionally restricted 7O Isl1-expressing cells overlap with Pax6-expressing cells.
Specimen 7P: embryonic day 12.5 (more )
Note: Double labeled: red - Isl1; green - Pax6 (assay MGI:7524731).
Structure Level Pattern Image Note
TS20: prethalamus Present 7P
Specimen 8J: embryonic day 10.5 (more )
Note: Double labeled: red - Isl1; green - BrdU.
Structure Level Pattern Image Note
TS17: diencephalon mantle layer Present Regionally restricted 8J Expression in ventral thalamus. Expression only in BrdU-negative cells.
Specimen 8K: embryonic day 10.5 (more )
Note: Double labeled: red - Isl1; green - Lhx1 (assay MGI:7524729) and Lhx5 (assay MGI:7524730).
Structure Level Pattern Image Note
TS17: diencephalon mantle layer Present Regionally restricted 8K Expression in ventral thalamus. Mantle zone composed of heterogeneous cell populations for expression of Isl1, Lhx1 and Pax6.
Specimen 8L: embryonic day 10.5 (more )
Note: Double labeled: red - Isl1; green - Pax6 (assay MGI:7524731).
Structure Level Pattern Image Note
TS17: diencephalon mantle layer Present Regionally restricted 8L Expression in ventral thalamus. Mantle zone composed of heterogeneous cell populations for expression of Isl1, Lhx1 and Pax6.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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