Probe:	ngn2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS27: dorsal thalamus	Present

Structure	Level	Pattern	Image	Note
TS24: lateral dorsal thalamic nucleus	Present		3J	At rostral levels, expression in laterodorsal nucleus.

Structure	Level	Pattern	Image	Note
TS24: dorsal lateral geniculate nucleus	Present		3K	At middle layer, square-shaped region expresses Ngn2, compatible with dorsal lateral geniculate nucleus.
TS24: lateral posterior thalamic nucleus	Trace		3K	Putative lateral posterior nucleus expressses only very low levels of Ngn2.
TS24: ventral posterior thalamic group	Weak		3K	Putative ventroposterior nucleus is weakly positive for Ngn2.
TS24: ventral medial thalamic nucleus	Present		3K	Putative ventromedial nucleus is positive for Ngn2.

Structure	Level	Pattern	Image	Note
TS24: medial geniculate nucleus	Weak	Regionally restricted	3L	At caudal level, ventral subdivision of medial geniculate nucleus is weaker for Ngn2. Dorsal subdivision of medial geniculate nucleus does not express Ngn2.
TS24: peripeduncular nucleus	Absent		3L	Putative peripeduncular nucleus is negative for Ngn2.

Structure	Level	Pattern	Image	Note
TS22: lateral dorsal thalamic nucleus	Present		4J	At rostral level, Ngn2 expressed in lateral-ventral part of dorsal thalamus, corresponding to laterodorsal nucleus.
TS22: dorsal thalamus	Present	Regionally restricted	4J	Expression is weak in band of Lhx2 and Gbx2 expression,
TS22: thalamus ventricular layer	Strong		4J	Strong expression in putative ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: thalamus ventricular layer	Strong		4K	Strong expression in putative ventricular zone.
TS22: dorsal thalamus	Present	Regionally restricted	4K	More caudally, region expressing Ngn2.
TS22: lateral posterior thalamic nucleus	Weak		4K	Low levels of Ngn2 expression in putative lateral posterior nucleus.
TS22: ventral posterior thalamic group	Weak		4K	Putative ventroposterior nucleus is weakly positive for Ngn2.

Structure	Level	Pattern	Image	Note
TS22: peripeduncular nucleus	Absent		4L	Putative peripeduncular nucleus is negative for Ngn2 expression.
TS22: medial geniculate nucleus	Weak	Regionally restricted	4L	Ventral subdivision of medial geniculate nucleus weakly expresses Ngn2.

Structure	Level	Pattern	Image	Note
TS20: thalamus mantle layer	Present		4S
TS20: thalamus ventricular layer	Present		4S
TS20: thalamus	Weak	Regionally restricted	4S	Weak expression in band with strong Lhx2/Gbx2 expression.

Structure	Level	Pattern	Image	Note
TS20: thalamus mantle layer	Present		4T
TS20: thalamus ventricular layer	Present		4T
TS20: thalamus	Weak	Regionally restricted	4T	Weak expression in band with strong Lhx2/Gbx2 expression.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8D	Expression in dorsal thalamus. No expression in lateral part in which Lhx2, Lhx9, and Gbx2 are expressed
TS17: diencephalon ventricular layer	Present		8D	Ngn2 expressed only in ventricular zone