Probe:	Gbx2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS27: central lateral thalamic nucleus	Present		1U

Structure	Level	Pattern	Image	Note
TS27: central lateral thalamic nucleus	Present		1V
TS27: central medial thalamic nucleus	Present		1V

Structure	Level	Pattern	Image	Note
TS27: lateral posterior thalamic nucleus	Present		1W

Structure	Level	Pattern	Image	Note
TS27: lateral posterior thalamic nucleus	Present		1X

Structure	Level	Pattern	Image	Note
TS27: medial geniculate nucleus	Present	Regionally restricted	1Y	Within medial geniculate nucleus, expressed most highly in ventral subdivision, especially in lateral part.

Structure	Level	Pattern	Image	Note
TS27: dorsal thalamus	Present	Regionally restricted		Expression in anteromedial nucleus, mediodorsal nucleus, paratenial nucleus and paraventricular nucleus. Expression not detected in dorsal lateral geniculate nucleus, posterior complex, ventrolateral nucleus, ventromedial nucleus and ventroposterior nucleus.
TS27: thalamic nucleus	Present	Regionally restricted		Expression in anteromedial nucleus, mediodorsal nucleus, paratenial nucleus and paraventricular nucleus. Expression not detected in dorsal lateral geniculate nucleus, posterior complex, ventrolateral nucleus, ventromedial nucleus and ventroposterior nucleus.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present	Regionally restricted	3G	At rostral level, Gbx2 is undetectable.

Structure	Level	Pattern	Image	Note
TS24: dorsal lateral geniculate nucleus	Present		3H	At middle level, square shaped region expresses Lhx9, compatible with dorsal lateral geniculate nucleus.
TS24: lateral posterior thalamic nucleus	Strong		3H	Putative lateral posterior nucleus expresses high levels of Gbx2.
TS24: ventral posterior thalamic group	Absent		3H	Putative ventroposterior nucleus is negative for Gbx2.
TS24: ventral medial thalamic nucleus	Absent		3H	Putative ventromedial nucleus is negative for Gbx2.

Structure	Level	Pattern	Image	Note
TS24: medial geniculate nucleus	Strong	Regionally restricted	3I	At caudal levels, ventral subdivision of medial geniculate nucleus is strongly positive for Gbx2. Dorsal subdivision of medial geniculate nucleus expresses Gbx2.
TS24: peripeduncular nucleus	Absent		3I	Putative peripeduncular nucleus is negative for Gbx2.

Structure	Level	Pattern	Image	Note
TS22: lateral dorsal thalamic nucleus	Absent		4G	At rostral level, Gbx2 not expressed in lateral-ventral part of dorsal thalamus, corresponding to laterodorsal nucleus.
TS22: dorsal thalamus	Strong	Regionally restricted	4G	Band of strong Gbx2 expression located more medially.

Structure	Level	Pattern	Image	Note
TS22: thalamus ventricular layer	Absent		4H	No expression in putative ventricular zone.
TS22: dorsal thalamus	Present	Regionally restricted	4H	More caudally, no expression in region expressing Lhx9 and Ngn2.
TS22: lateral posterior thalamic nucleus	Strong		4H	High levels of Gbx2 expression in putative lateral posterior nucleus.

Structure	Level	Pattern	Image	Note
TS22: peripeduncular nucleus	Absent		4I	Putative peripeduncular nucleus is negative for Gbx2 expression.
TS22: medial geniculate nucleus	Strong	Regionally restricted	4I	Ventral subdivision of medial geniculate nucleus strongly expresses Gbx2.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4Q	Band with high levels of Gbx2 expression located medial to band with high Lhx9 expression.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4R	Band with high levels of Gbx2 expression located medial to band with high Lhx9 expression.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8C	Expression in lateral part of dorsal thalamus.