Probe:	Pomc probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	4a
TS20: cartilage	Present		4a
TS20: head mesenchyme	Present		4a
TS20: skeletal muscle	Present		4a
TS20: gut	Strong		4a
TS20: limb musculature	Strong		4a
TS20: mandibular process mesenchyme	Strong		4a
TS20: rib pre-cartilage condensation	Strong		4a
TS20: trunk musculature	Strong	Regionally restricted	4a	Expression was strong in intercostal muscle masses.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong		4b
TS21: lung mesenchyme	Strong		4b
TS21: peripheral nervous system	Strong		4b
TS21: spinal cord	Strong		4b
TS21: lung epithelium	Present		4b

Structure	Level	Pattern	Image	Note
TS21: dermis	Strong		4c
TS21: liver	Strong		4c
TS21: trunk musculature	Present	Regionally restricted	4c	Expression was detected in epaxial skeletal muscle.
TS21: diaphragm	Present	Regionally restricted	4c	Expression was detected in diaphragmatic smooth muscle.
TS21: intestine smooth muscle	Present		4c
TS21: vertebral cartilage condensation	Weak		4c
TS21: stomach epithelium	Present		4c

Structure	Level	Pattern	Image	Note
TS21: head	Present		4d
TS21: heart	Present		4d
TS21: pituitary gland	Present		4d
TS21: salivary gland epithelium	Present		4d
TS21: cerebellum	Strong		4d
TS21: hypothalamus	Strong		4d
TS21: nasal epithelium	Strong		4d
TS21: pons	Strong		4d
TS21: tongue	Strong		4d

Structure	Cell Type	Level	Pattern	Image	Note
TS25: facial muscle		Present		4f
TS25: nasal epithelium		Present		4f
TS25: nasal gland		Present		4f
TS25: vibrissa		Present		4f
TS25: hair follicle		Present	Regionally restricted	4f	Expression was detected in standard-sized hair follicles of the face.
TS25: neural retina		Strong		4f
TS25: optic nerve	glial cell	Present		4f

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Strong		4g
TS25: hippocampus	Strong		4g