Antibody:	anti-Msi1
Detection system:	Alexa Fluor

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		5B	Expression in neuroepithelium. Co-expression with Msi2 in most cells.

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Present		5H	Co-expression with Msi2.

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Present	Regionally restricted	5H inset	Cytoplasmic expression in neuroepithelial cells. Co-expression with Msi2.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence ventricular zone	Present		5J	Expression co-localized with Msi2 in ventricular zone of telencephalon and ganglionic eminences.
TS22: telencephalon ventricular layer	Present		5J	Expression co-localized with Msi2 in ventricular zone of telencephalon and ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS28: telencephalon subventricular zone	Present		5N	Expression in many densely packed cells in subventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: telencephalon subventricular zone		Present		5P	Expression in many densely packed cells in subventricular zone.
TS28: lateral ventricle ependyma		Present		5P	Expression in many densely packed cells in ependymal layer.
TS28: cerebral cortex	astrocyte	Present	Scattered	5P	Expression in small scattered astrocytes in parenchyma.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Uniform	6B	Expression uniformaly distributed with neural tube. Co-expression with Msi2 in most cells.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		6F	Co-expression with Msi2.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present		6J	Simulaneously expressed with Msi1 in ventricular zone lining central canal.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present		6L	Co-expression with Msi2.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present		6N

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex	astrocyte	Present	Widespread	8B	Expression in many astrocytes with small bodies throughout cortex.
TS28: cortical layer II	neuron	Absent		8B	No expression in subset of Msi2-positive neurons with larger cell bodies found sparcely in layers II-III.
TS28: cortical layer III	neuron	Absent		8B	No expression in subset of Msi2-positive neurons with larger cell bodies found sparcely in layers II-III.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: telencephalon	astrocyte	Present	Regionally restricted	8F	Expression in Gfap-negative protoplasmic astrocytes frequently observed in deep gray matter.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer		Strong	Regionally restricted	10A	Intense expression in neuronal precursor cells in external granule layer.
TS27: cerebellum Purkinje cell layer	Purkinje cell	Absent		10A	No expression in large Purkinje neurons in Purkinje cell layer.
TS27: cerebellum white matter	glial restricted tripotential precursor cell	Present	Regionally restricted	10A	Expression in numerous glial precursor cells in folia white matter.
TS27: deep cerebellar nucleus		Absent		10A	No expression in deep cerebellar nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum white matter	astrocyte	Strong		10C	Highly expressed in astrocytes in central white matter tracts.
TS28: cerebellum internal granule cell layer		Strong		10C	Highly expressed in astrocytes in internal granule cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		10E	No expression in Purkinje cells in Purkinje cell layer
TS28: cerebellum	Bergmann glial cell	Present		10E	Expression in many small cells in vicinity of Purkinje cells - likely to be developing Bergmann glia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		10I	No expression in Purkinje cells in Purkinje cell layer
TS28: cerebellum	Bergmann glial cell	Present		10I	Expression in soma of Bergman glial cells and their fibers coursing from Purkinje cell layer through molecular layer to pial surface.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	astrocyte	Present			Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.
TS28: medial preoptic nucleus	astrocyte	Present			Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.
TS28: supraoptic nucleus	astrocyte	Present			Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.