Gene Expression Data
Assay Details
Assay
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Reference:
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J:72036
Sakakibara S, et al., Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. J Neurosci. 2001 Oct 15;21(20):8091-107
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7522281
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Gene symbol:
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Msi1
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Gene name:
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musashi RNA-binding protein 1
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Antibody:
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anti-Msi1
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Detection system:
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Alexa Fluor
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Results
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Specimen
5B:
embryonic day 10.5
(more )
Specimen
5B:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Present |
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5B
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Expression in neuroepithelium. Co-expression with Msi2 in most cells.
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Specimen
5H:
embryonic day 12.5
(more )
Specimen
5H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5H inset:
embryonic day 12.5
(more )
Specimen
5H inset:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5J:
embryonic day 14.5
(more )
Specimen
5J:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: ganglionic eminence |
Present |
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5J
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Expression co-localized with Msi2 in ventricular zone of telencephalon and ganglionic eminences. Co-expression with Msi2.
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TS22: telencephalon ventricular layer |
Present |
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5J
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Expression co-localized with Msi2 in ventricular zone of telencephalon and ganglionic eminences. Co-expression with Msi2.
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Specimen
5N:
postnatal day 7
(more )
Specimen
5N:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5P:
postnatal adult
(more )
Specimen
5P:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
embryonic day 10.5
(more )
Specimen
6B:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Uniform |
6B
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Expression uniformaly distributed with neural tube. Co-expression with Msi2 in most cells.
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Specimen
6F:
embryonic day 12.5
(more )
Specimen
6F:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6J:
embryonic day 14.5
(more )
Specimen
6J:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 14.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6L:
embryonic day 14.5
(more )
Specimen
6L:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6N:
postnatal adult
(more )
Specimen
6N:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8B:
postnatal adult
(more )
Specimen
8B:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
astrocyte |
Present |
Widespread |
8B
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Expression in many astrocytes with small bodies throughout cortex.
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TS28: cortical layer II |
neuron |
Absent |
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8B
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No expression in subset of Msi2-positive neurons with larger cell bodies found sparcely in layers II-III.
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TS28: cortical layer III |
neuron |
Absent |
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8B
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No expression in subset of Msi2-positive neurons with larger cell bodies found sparcely in layers II-III.
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Specimen
8F:
postnatal adult
(more )
Specimen
8F:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
astrocyte |
Present |
Regionally restricted |
8F
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Expression in Gfap-negative protoplasmic astrocytes frequently observed in deep gray matter.
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Specimen
10A:
postnatal day 3
(more )
Specimen
10A:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
10C:
postnatal adult
(more )
Specimen
10C:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
10E:
postnatal adult
(more )
Specimen
10E:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebellum Purkinje cell layer |
Purkinje cell |
Absent |
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10E
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No expression in Purkinje cells in Purkinje cell layer
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TS28: cerebellum |
Bergmann glial cell |
Present |
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10E
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Expression in many small cells in vicinity of Purkinje cells - likely to be developing Bergmann glia.
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Specimen
10I:
postnatal adult
(more )
Specimen
10I:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebellum Purkinje cell layer |
Purkinje cell |
Absent |
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10I
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No expression in Purkinje cells in Purkinje cell layer
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TS28: cerebellum |
Bergmann glial cell |
Present |
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10I
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Expression in soma of Bergman glial cells and their fibers coursing from Purkinje cell layer through molecular layer to pial surface.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lateral habenular nucleus |
astrocyte |
Present |
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Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.
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TS28: medial preoptic nucleus |
astrocyte |
Present |
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Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.
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TS28: supraoptic nucleus |
astrocyte |
Present |
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Expression in astrocytes in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and medial and lateral habeular nucleus.
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