Antibody:	anti-Neuropilin-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present		1B	Expression is in axons extend into the lateral nerve layer of the rostral olfactory bulb.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb	Present	Regionally restricted	1D	Expression is in axons that track ventrally in the central olfactory bulb.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present		1H , 1F	Expression is in axons that grow through the ventral nerve layer into the medial nerve layer in the caudal olfactory bulb

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	2B	Expression is in axons that grow rostrally and caudally in regions where Sema3a is absent.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	2D	Expression is in the medial and ventral nerve layer.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present	Regionally restricted	4A	Expression is in axons that grow laterally into all nerve layer regions.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present	Regionally restricted	4B	Positive axons grow diffusely into the dorsolateral and ventrolateral nerve layer in the rostral olfactory bulb.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present	Regionally restricted	4C	Expression is in axons that disperse into the medial and ventro-lateral nerve layer.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Present	Regionally restricted	4D	Positive axons can only be found in the ventral nerve layer compared to wild-type.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Strong	Regionally restricted	4E	Expression is in the axon bundle in the nerve layer of the medial olfactory bulb.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Weak	Regionally restricted	4F	Expression is in the ventral nerve layer.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5A	Expression is in axons that grow laterally into the nerve layer.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5B	Positive axons are detectable in most regions.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5C , 5E	Positive axons grow laterally and to a lesser extent medially.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5D , 5F	Positive axon trajectories extend into the ventral and medial olfactory bulb.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5G	Positive axons grow medially.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	5H	Expression in this mutant is the same as in wild-type in this area.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	6A	Expression is in axons that grow laterally into the nerve layer.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	6B	Positive axons are dispersed into all regions.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	6C	Expression is in axons that grow laterally into the nerve layer. Expression is in small protoglomeruli.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb layer	Present	Regionally restricted	6D	Positive axons and protoglomeruli are uncharacteristically present in the ventral olfactory bulb.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted		Expression is in axons in the olfactory bulb.