Antibody:	anti-AP-2b
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Structure	Level	Pattern	Image	Note
TS17: rhombomere 1	Present	Regionally restricted	1B	Not expressed in the rhombic lip and the intermediate domain, where the first cerebellar neurons were generated.

Structure	Level	Pattern	Image	Note
TS20: cerebellum white matter	Present	Regionally restricted	1D	Expressed in GABAergic precursors in the underlying white matter where inhibitory neurons transit after exiting the ventricular zone. Expression was more widespread than Tfap2a, may reflect the expression in non-cerebellar cell types.

Structure	Level	Pattern	Image	Note
TS23: cerebellum white matter	Present	Regionally restricted	1F	Expressed in GABAergic precursors in the underlying white matter where inhibitory neurons transit after exiting the ventricular zone. Expression was more widespread than Tfap2a, may reflect the expression in non-cerebellar cell types.

Structure	Level	Pattern	Image	Note
TS27: cerebellum white matter	Present		1H
TS27: cerebellum Purkinje cell layer	Absent		1H

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present	Regionally restricted	1J	Expressed in the lower half.
TS28: cerebellum molecular layer	Present	Regionally restricted	1J	Expressed in the lower half.
TS28: cerebellum Purkinje cell layer	Absent		1J

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		1L
TS28: cerebellum molecular layer	Present		1L
TS28: cerebellum Purkinje cell layer	Absent		1L