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Gene Expression Data
Assay Details
Assay
Reference: J:317244 Lee J, et al., Fetal hematopoietic stem cells express MFG-E8 during mouse embryogenesis. Exp Mol Med. 2015 Jul 24;47:e174
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7514399
Gene symbol: Mfge8
Gene name: milk fat globule EGF and factor V/VIII domain containing
Antibody: anti-MFG-E8
Detection system: Secondary antibody
Results
Specimen 1d/f/g: embryonic day 10.5 (more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Structure Cell Type Level Pattern Image Note
TS17: blood hematopoietic stem cell Strong Regionally restricted 1d , 1f , 1g Co-expressed with Cd34 in large cells with prominent nuclei in the yolk sac blood islands as aggregates.
TS17: yolk sac mesenchyme hematopoietic stem cell Strong Regionally restricted 1d , 1f , 1g Co-expressed with Cd34 in large cells with prominent nuclei in the yolk sac blood islands as aggregates.
Specimen 2b/d: embryonic day 11.5 (more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Structure Cell Type Level Pattern Image Note
TS19: blood hematopoietic stem cell Present Regionally restricted 2b , 2d Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
TS19: cardinal vein hematopoietic stem cell Present Regionally restricted 2b , 2d Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
TS19: dorsal aorta hematopoietic stem cell Present Regionally restricted 2b , 2d Co-expressed with Cd34 in the dorsal aorta and cardinal veins region.
Specimen 2f/h: embryonic day 11.5 (more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Structure Cell Type Level Pattern Image Note
TS19: blood hematopoietic stem cell Present Regionally restricted 2f , 2h Co-expressed with Cd34 in many prominent colonies of eosinophilic and nucleate cells over the entire fetal liver parenchyma.
TS19: liver parenchyma hematopoietic stem cell Present Regionally restricted 2f , 2h Co-expressed with Cd34 in many prominent colonies of eosinophilic and nucleate cells over the entire fetal liver parenchyma.
Specimen 3b/d: embryonic day 11.5 (more )
Note: Double labeled: green - Mfge8; red - Cd34 (assay MGI:7514400).
Structure Cell Type Level Pattern Image Note
TS19: blood hematopoietic stem cell Present Regionally restricted 3d , 3b Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
TS19: chorionic plate hematopoietic stem cell Present Regionally restricted 3d , 3b Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
TS19: labyrinthine zone hematopoietic stem cell Present Regionally restricted 3d , 3b Co-expressed with Cd34 in a number of cells with large nuclei in large fetal blood vessels in the chorioallantoic mesenchyme.
Specimen 5b: postnatal adult (more )
Note: Double labeled: green - Mfge8; red - Adgre1 (assay MGI:7514403).
Structure Cell Type Level Pattern Image Note
TS28: bone marrow bone marrow macrophage Present 5b MFG-E8 , 5b merge Co-expressed with Adgre1 (F4/80).
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: decidua Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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